Open Access
Open access
Springer Nature
Clinical Proteomics
,  volume 21 ,  issue 1 ,  publication number 50

ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis

Qing Yu 1, 2
, 
Chunhua Qu 3
, 
Jinliang Liang 4
, 
Peiqi Chen 3
, 
Kaiying Zhang 3
, 
Yanji Zhang 3
, 
Yuening Zhang 5
, 
Li Zherui 6
, 
Shaojun Liu 7
, 
Zhaoshou Yang 8
, 
Hongyan Sun 3
, 
Anli Yang 3
1
 
Department of Clinical Laboratory, Shenzhen Kangning Hospital, Shenzhen, P. R. China | 
2
 
Department of Clinical Laboratory, Maternal & Child Health Hospital of Foshan, Foshan, P.R. China | 
3
 
4
 
5
 
6
 
7
 
8
 
Publication type: Journal Article
Publication date: 2024-07-15
Springer Nature
scimago Q1
wos Q2
SJR: 0.962
CiteScore: 4.3
Impact factor: 3.3
ISSN: 15426416, 15590275
Abstract
Background

Estrogen receptor-positive (ER+) breast cancer accounts for two-thirds of all breast cancers, and its early and late recurrences still threaten patients’ long-term survival and quality of life. Finding candidate tumor antigens and potential therapeutic targets is critical to addressing these unmet needs.

Method

The isobaric tags for relative and absolute quantitation (iTRAQ) proteomic analysis was employed to identify the differentially expressed proteins (DEPs) between ER + breast cancer and corresponding adjacent normal tissue. Candidate DEPs were screened by bioinformatic analyses, and their expression was confirmed by immunohistochemical (IHC) staining and western blot. A series of in vitro experiments, including wound healing assay, colony formation, and cell cycle assay, were performed to reveal the functions of selected DEPs. Additionally, their clinical significances were further analyzed.

Result

A total of 369 DEPs (fold change ≥ 2.0 or ≤ 0.66, P < 0.05) were discovered. Compared with normal tissue, 358 proteins were up-regulated and 11 proteins were down-regulated in ER + breast cancer. GO and KEGG enrichment analysis showed that DEPs were closely associated with RNA regulation and metabolic pathways. STRING analysis found ESF1 and MIPEP were the hub genes in breast cancer, whose increased expressions were verified by the IHC staining and western blot. Knocking down ESF1 and MIPEP inhibited colony formation and increased cell apoptosis. Besides, knocking down ESF1 inhibited wound healing but not MIPEP. In addition, ESF1 and MIPEP expression were negatively associated with patient prognosis.

Conclusion

The upregulation of ESF1 and MIPEP promoted ER + breast cancer proliferation, which might provide novel targets for the development of new therapies.

Found 

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Yu Q. et al. ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis // Clinical Proteomics. 2024. Vol. 21. No. 1. 50
GOST all authors (up to 50) Copy
Yu Q., Qu C., Liang J., Chen P., Zhang K., Zhang Y., Zhang Y., Zherui L., Liu S., Yang Z., Sun H., Yang A. ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis // Clinical Proteomics. 2024. Vol. 21. No. 1. 50
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RIS Copy
TY - JOUR
DO - 10.1186/s12014-024-09502-8
UR - https://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-024-09502-8
TI - ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis
T2 - Clinical Proteomics
AU - Yu, Qing
AU - Qu, Chunhua
AU - Liang, Jinliang
AU - Chen, Peiqi
AU - Zhang, Kaiying
AU - Zhang, Yanji
AU - Zhang, Yuening
AU - Zherui, Li
AU - Liu, Shaojun
AU - Yang, Zhaoshou
AU - Sun, Hongyan
AU - Yang, Anli
PY - 2024
DA - 2024/07/15
PB - Springer Nature
IS - 1
VL - 21
PMID - 39004717
SN - 1542-6416
SN - 1559-0275
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Yu,
author = {Qing Yu and Chunhua Qu and Jinliang Liang and Peiqi Chen and Kaiying Zhang and Yanji Zhang and Yuening Zhang and Li Zherui and Shaojun Liu and Zhaoshou Yang and Hongyan Sun and Anli Yang},
title = {ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis},
journal = {Clinical Proteomics},
year = {2024},
volume = {21},
publisher = {Springer Nature},
month = {jul},
url = {https://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-024-09502-8},
number = {1},
pages = {50},
doi = {10.1186/s12014-024-09502-8}
}