Open Access
BMC Genomics, volume 16, issue 1, publication number 965
Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats
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Quantitative Proteomics, Institute of Molecular Biology (IMB) gGmbH, Mainz, Germany
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Publication type: Journal Article
Publication date: 2015-11-17
PubMed ID:
26577093
Genetics
Biotechnology
Abstract
BackgroundTo date, telomere research in fungi has mainly focused on Saccharomyces cerevisiae and Schizosaccharomyces pombe, despite the fact that both yeasts have degenerated telomeric repeats in contrast to the canonical TTAGGG motif found in vertebrates and also several other fungi.ResultsUsing label-free quantitative proteomics, we here investigate the telosome of Neurospora crassa, a fungus with canonical telomeric repeats. We show that at least six of the candidates detected in our screen are direct TTAGGG-repeat binding proteins. While three of the direct interactors (NCU03416 [ncTbf1], NCU01991 [ncTbf2] and NCU02182 [ncTay1]) feature the known myb/homeobox DNA interaction domain also found in the vertebrate telomeric factors, we additionally show that a zinc-finger protein (NCU07846) and two proteins without any annotated DNA-binding domain (NCU02644 and NCU05718) are also direct double-strand TTAGGG binders. We further find two single-strand binders (NCU02404 [ncGbp2] and NCU07735 [ncTcg1]).ConclusionBy quantitative label-free interactomics we identify TTAGGG-binding proteins in Neurospora crassa, suggesting candidates for telomeric factors that are supported by phylogenomic comparison with yeast species. Intriguingly, homologs in yeast species with degenerated telomeric repeats are also TTAGGG-binding proteins, e.g. in S. cerevisiae Tbf1 recognizes the TTAGGG motif found in its subtelomeres. However, there is also a subset of proteins that is not conserved. While a rudimentary core TTAGGG-recognition machinery may be conserved across yeast species, our data suggests Neurospora as an emerging model organism with unique features.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Casas Vila N. et al. Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats // BMC Genomics. 2015. Vol. 16. No. 1. 965
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Casas Vila N., Scheibe M., Freiwald A., Kappei D., Butter F. Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats // BMC Genomics. 2015. Vol. 16. No. 1. 965
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TY - JOUR
DO - 10.1186/s12864-015-2158-0
UR - https://doi.org/10.1186%2Fs12864-015-2158-0
TI - Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats
T2 - BMC Genomics
AU - Casas Vila, Núria
AU - Scheibe, Marion
AU - Freiwald, Anja
AU - Kappei, Dennis
AU - Butter, Falk
PY - 2015
DA - 2015/11/17 00:00:00
PB - Springer Nature
IS - 1
VL - 16
PMID - 26577093
SN - 1471-2164
ER -
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@article{2015_Casas Vila,
author = {Núria Casas Vila and Marion Scheibe and Anja Freiwald and Dennis Kappei and Falk Butter},
title = {Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats},
journal = {BMC Genomics},
year = {2015},
volume = {16},
publisher = {Springer Nature},
month = {nov},
url = {https://doi.org/10.1186%2Fs12864-015-2158-0},
number = {1},
doi = {10.1186/s12864-015-2158-0}
}