Open Access
AMB Express, volume 12, issue 1, publication number 105
Ursodeoxycholic acid production by Gibberella zeae mutants
Publication type: Journal Article
Publication date: 2022-08-08
Biophysics
Applied Microbiology and Biotechnology
Abstract
Ursodeoxycholic acid (UDCA) is a highly demanded pharmaceutical steroid widely used in medicine. An ascomycete Gibberella zeae VKM F-2600 is capable of producing UDCA by 7β-hydroxylation of lithocholic acid (LCA). The present study is aimed at the improvement of the fungus productivity. The original procedures for the protoplast obtaining followed by UV mutagenesis and screening of ketoconazole-resistant mutant clones have been applied. The highest yield of G. zeae protoplasts was obtained when using the mycelium in the active growth phase, ammonium chloride as an osmotic stabilizer and treatment of the fungal cells by the lytic enzymes cocktail from Trichoderma hurzanium. The conditions for effective protoplast regeneration and the UV-mutagenesis were found to provide 6–12% survival rate of the protoplasts with superior number of possible mutations. Three of 27 ketoconazole-resistant mutant clones obtained have been selected due to their increased biocatalytic activity towards LCA. The mutant G. zeae M23 produced 26% more UDCA even at relatively high LCA concentration (4 g/L) as compared with parent fungal strain, and the conversion reached 88% (w/w). The yield of UDCA reached in this study prefers those ever reported. The results contribute to the knowledge on ascomycete mutagenesis, and are of importance for biotechnological production of value added cholic acids.
Citations by journals
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1
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Frontiers in Microbiology
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Frontiers in Microbiology
1 publication, 50%
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Journal of Steroid Biochemistry and Molecular Biology
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Journal of Steroid Biochemistry and Molecular Biology
1 publication, 50%
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1
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Citations by publishers
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1
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 50%
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Elsevier
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Elsevier
1 publication, 50%
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1
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Kollerov V., Donova M. Ursodeoxycholic acid production by Gibberella zeae mutants // AMB Express. 2022. Vol. 12. No. 1. 105
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Kollerov V., Donova M. Ursodeoxycholic acid production by Gibberella zeae mutants // AMB Express. 2022. Vol. 12. No. 1. 105
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TY - JOUR
DO - 10.1186/s13568-022-01446-2
UR - https://doi.org/10.1186%2Fs13568-022-01446-2
TI - Ursodeoxycholic acid production by Gibberella zeae mutants
T2 - AMB Express
AU - Kollerov, Vyacheslav
AU - Donova, Marina
PY - 2022
DA - 2022/08/08 00:00:00
PB - Springer Nature
IS - 1
VL - 12
SN - 2191-0855
ER -
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@article{2022_Kollerov,
author = {Vyacheslav Kollerov and Marina Donova},
title = {Ursodeoxycholic acid production by Gibberella zeae mutants},
journal = {AMB Express},
year = {2022},
volume = {12},
publisher = {Springer Nature},
month = {aug},
url = {https://doi.org/10.1186%2Fs13568-022-01446-2},
number = {1},
doi = {10.1186/s13568-022-01446-2}
}