Open Access
Open access
F1000 Research
F1000Research
,  volume 2 ,  pages 216

Buffering the pH of the culture medium does not extend yeast replicative lifespan

Brian M Wasko 1
, 
Daniel T Carr 1
, 
Herman Tung 1
, 
Ha Doan 1
, 
Nathan Schurman 2
, 
Jillian R Neault 1
, 
Joey Feng 1
, 
Janet Lee 1
, 
Janet S. Lee 2
, 
Ben Zipkin 1
, 
Jacob Mouser 1
, 
Edward Oudanonh 1
, 
Tina Nguyen 2
, 
Torin Stetina 1
, 
Anna Shemorry 1
, 
Mekedes Lemma 1
, 
Matt Kaeberlein 1
1
 
2
 
Publication type: Journal Article
Publication date: 2013-10-15
F1000 Research
scimago Q1
SJR: 0.537
CiteScore: 2.9
Impact factor: —
ISSN: 20461402
General Biochemistry, Genetics and Molecular Biology
General Medicine
General Immunology and Microbiology
General Pharmacology, Toxicology and Pharmaceutics
Abstract
During chronological aging of budding yeast cells, the culture medium can become acidified, and this acidification limits cell survival.  As a consequence, buffering the culture medium to pH 6 significantly extends chronological life span under standard conditions in synthetic medium.  In this study, we assessed whether a similar process occurs during replicative aging of yeast cells.  We find no evidence that buffering the pH of the culture medium to pH levels either higher or lower than the initial pH of the medium is able to significantly extend replicative lifespan.  Thus, we conclude that, unlike chronological life span, replicative life span is not limited by acidification of the culture medium or by changes in the pH of the environment.
Found 
Found 

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GOST Copy
Wasko B. M. et al. Buffering the pH of the culture medium does not extend yeast replicative lifespan // F1000Research. 2013. Vol. 2. p. 216.
GOST all authors (up to 50) Copy
Wasko B. M., Carr D. T., Tung H., Doan H., Schurman N., Neault J. R., Feng J., Lee J., Lee J. S., Zipkin B., Mouser J., Oudanonh E., Nguyen T., Stetina T., Shemorry A., Lemma M., Kaeberlein M. Buffering the pH of the culture medium does not extend yeast replicative lifespan // F1000Research. 2013. Vol. 2. p. 216.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.12688/f1000research.2-216.v1
UR - https://f1000research.com/articles/2-216/v1
TI - Buffering the pH of the culture medium does not extend yeast replicative lifespan
T2 - F1000Research
AU - Wasko, Brian M
AU - Carr, Daniel T
AU - Tung, Herman
AU - Doan, Ha
AU - Schurman, Nathan
AU - Neault, Jillian R
AU - Feng, Joey
AU - Lee, Janet
AU - Lee, Janet S.
AU - Zipkin, Ben
AU - Mouser, Jacob
AU - Oudanonh, Edward
AU - Nguyen, Tina
AU - Stetina, Torin
AU - Shemorry, Anna
AU - Lemma, Mekedes
AU - Kaeberlein, Matt
PY - 2013
DA - 2013/10/15
PB - F1000 Research
SP - 216
VL - 2
PMID - 24555104
SN - 2046-1402
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2013_Wasko,
author = {Brian M Wasko and Daniel T Carr and Herman Tung and Ha Doan and Nathan Schurman and Jillian R Neault and Joey Feng and Janet Lee and Janet S. Lee and Ben Zipkin and Jacob Mouser and Edward Oudanonh and Tina Nguyen and Torin Stetina and Anna Shemorry and Mekedes Lemma and Matt Kaeberlein},
title = {Buffering the pH of the culture medium does not extend yeast replicative lifespan},
journal = {F1000Research},
year = {2013},
volume = {2},
publisher = {F1000 Research},
month = {oct},
url = {https://f1000research.com/articles/2-216/v1},
pages = {216},
doi = {10.12688/f1000research.2-216.v1}
}