Open Access
Optics Express, volume 13, issue 18, pages 7052
Superresolution by localization of quantum dots using blinking statistics
Publication type: Journal Article
Publication date: 2005-09-29
Journal:
Optics Express
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor: 3.8
ISSN: 10944087, 10944087
Atomic and Molecular Physics, and Optics
Abstract
In microscopy, single fluorescence point sources can be localized with a precision several times greater than the resolution limit of the microscope. We show that the intermittent fluorescence or ‘blinking’ of quantum dots can analyzed by an Independent Component Analysis so as to identify the light emitted by each individual nanoparticle, localize it precisely, and thereby resolve groups of closely spaced (< λ/30) quantum dots. Both simulated and experimental data demonstrate that this technique is superior to localization based on Maximum Likelihood Estimation of the sum image under the assumption of point emitters. This technique has general application to any emitter with non-Gaussian temporal intensity distribution, including triplet state blinking. When applied to the labeling of structures, a high resolution “image” consisting of individually localized points may be reconstructed leading to the term “Pointillism”.
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Lidke K. A. et al. Superresolution by localization of quantum dots using blinking statistics // Optics Express. 2005. Vol. 13. No. 18. p. 7052.
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Lidke K. A., Rieger B., Jovin T. M., Heintzmann R. Superresolution by localization of quantum dots using blinking statistics // Optics Express. 2005. Vol. 13. No. 18. p. 7052.
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TY - JOUR
DO - 10.1364/opex.13.007052
UR - https://doi.org/10.1364/opex.13.007052
TI - Superresolution by localization of quantum dots using blinking statistics
T2 - Optics Express
AU - Lidke, Keith A.
AU - Rieger, Bernd
AU - Jovin, Thomas M.
AU - Heintzmann, Rainer
PY - 2005
DA - 2005/09/29 00:00:00
PB - Optical Society of America
SP - 7052
IS - 18
VL - 13
SN - 1094-4087
SN - 1094-4087
ER -
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@article{2005_Lidke,
author = {Keith A. Lidke and Bernd Rieger and Thomas M. Jovin and Rainer Heintzmann},
title = {Superresolution by localization of quantum dots using blinking statistics},
journal = {Optics Express},
year = {2005},
volume = {13},
publisher = {Optical Society of America},
month = {sep},
url = {https://doi.org/10.1364/opex.13.007052},
number = {18},
pages = {7052},
doi = {10.1364/opex.13.007052}
}
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MLA
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Lidke, Keith A., et al. “Superresolution by localization of quantum dots using blinking statistics.” Optics Express, vol. 13, no. 18, Sep. 2005, p. 7052. https://doi.org/10.1364/opex.13.007052.