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том 15 издание 1 страницы 114

Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising

Wentao Wu
Christoph Brandt
Xin Zhou
Shuo Tang
Тип публикацииJournal Article
Дата публикации2023-12-06
scimago Q1
wos Q1
БС1
SJR0.896
CiteScore6.4
Impact factor3.2
ISSN21567085
Biotechnology
Atomic and Molecular Physics, and Optics
Краткое описание

We report on a compact multimodal imaging system that can acquire two-photon microscopy (2PM) and three-photon microscopy (3PM) images simultaneously. With dual excitation wavelengths, multiple contrasts including two-photon-excitation-fluorescence (2PEF), second harmonic generation (SHG), and third harmonic generation (THG) are acquired simultaneously from cells, collagen fibers, and interfaces, all label-free. Challenges related to the excitation by two wavelengths and the effective separation of 2PM and 3PM signals are discussed and addressed. The data processing challenge where multiple contrasts can have significantly varying signal levels is also addressed. A kernel-based nonlinear scaling (KNS) denoising method is introduced to reduce noise from ultra-low signal images and generate high-quality multimodal images. Simultaneous 2PM and 3PM imaging is demonstrated on various tissue samples. The simultaneous acquisition speeds up the imaging process and minimizes the commonly encountered problem of motion artifacts and mechanical drift in sequential acquisition. Multimodal imaging with simultaneous 2PM and 3PM will have great potential for label-free in-vivo imaging of biological tissues.

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Laser and Photonics Reviews
2 публикации, 22.22%
Biomedical Optics Express
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Journal of Biophotonics
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Advanced Photonics Research
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Mendeleev Communications
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Optical Properties of Metal Oxide Nanostructures
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Optics Express
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Wiley
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Optica Publishing Group
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OOO Zhurnal "Mendeleevskie Soobshcheniya"
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Cold Spring Harbor Laboratory
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ГОСТ |
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Wu W. et al. Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising // Biomedical Optics Express. 2023. Vol. 15. No. 1. p. 114.
ГОСТ со всеми авторами (до 50) Скопировать
Wu W., Brandt C., Zhou X., Tang S. Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising // Biomedical Optics Express. 2023. Vol. 15. No. 1. p. 114.
RIS |
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TY - JOUR
DO - 10.1364/boe.504550
UR - https://doi.org/10.1364/boe.504550
TI - Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising
T2 - Biomedical Optics Express
AU - Wu, Wentao
AU - Brandt, Christoph
AU - Zhou, Xin
AU - Tang, Shuo
PY - 2023
DA - 2023/12/06
PB - Optica Publishing Group
SP - 114
IS - 1
VL - 15
PMID - 38223188
SN - 2156-7085
ER -
BibTex |
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BibTex (до 50 авторов) Скопировать
@article{2023_Wu,
author = {Wentao Wu and Christoph Brandt and Xin Zhou and Shuo Tang},
title = {Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising},
journal = {Biomedical Optics Express},
year = {2023},
volume = {15},
publisher = {Optica Publishing Group},
month = {dec},
url = {https://doi.org/10.1364/boe.504550},
number = {1},
pages = {114},
doi = {10.1364/boe.504550}
}
MLA
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Wu, Wentao, et al. “Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising.” Biomedical Optics Express, vol. 15, no. 1, Dec. 2023, p. 114. https://doi.org/10.1364/boe.504550.