Open Access
Open access
volume 15 issue 1 pages 114

Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising

Wentao Wu
Christoph Brandt
Xin Zhou
Shuo Tang
Publication typeJournal Article
Publication date2023-12-06
scimago Q1
wos Q1
SJR0.896
CiteScore6.4
Impact factor3.2
ISSN21567085
PubMed ID:  38223188
Biotechnology
Atomic and Molecular Physics, and Optics
Abstract

We report on a compact multimodal imaging system that can acquire two-photon microscopy (2PM) and three-photon microscopy (3PM) images simultaneously. With dual excitation wavelengths, multiple contrasts including two-photon-excitation-fluorescence (2PEF), second harmonic generation (SHG), and third harmonic generation (THG) are acquired simultaneously from cells, collagen fibers, and interfaces, all label-free. Challenges related to the excitation by two wavelengths and the effective separation of 2PM and 3PM signals are discussed and addressed. The data processing challenge where multiple contrasts can have significantly varying signal levels is also addressed. A kernel-based nonlinear scaling (KNS) denoising method is introduced to reduce noise from ultra-low signal images and generate high-quality multimodal images. Simultaneous 2PM and 3PM imaging is demonstrated on various tissue samples. The simultaneous acquisition speeds up the imaging process and minimizes the commonly encountered problem of motion artifacts and mechanical drift in sequential acquisition. Multimodal imaging with simultaneous 2PM and 3PM will have great potential for label-free in-vivo imaging of biological tissues.

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GOST |
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GOST Copy
Wu W. et al. Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising // Biomedical Optics Express. 2023. Vol. 15. No. 1. p. 114.
GOST all authors (up to 50) Copy
Wu W., Brandt C., Zhou X., Tang S. Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising // Biomedical Optics Express. 2023. Vol. 15. No. 1. p. 114.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1364/boe.504550
UR - https://doi.org/10.1364/boe.504550
TI - Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising
T2 - Biomedical Optics Express
AU - Wu, Wentao
AU - Brandt, Christoph
AU - Zhou, Xin
AU - Tang, Shuo
PY - 2023
DA - 2023/12/06
PB - Optica Publishing Group
SP - 114
IS - 1
VL - 15
PMID - 38223188
SN - 2156-7085
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2023_Wu,
author = {Wentao Wu and Christoph Brandt and Xin Zhou and Shuo Tang},
title = {Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising},
journal = {Biomedical Optics Express},
year = {2023},
volume = {15},
publisher = {Optica Publishing Group},
month = {dec},
url = {https://doi.org/10.1364/boe.504550},
number = {1},
pages = {114},
doi = {10.1364/boe.504550}
}
MLA
Cite this
MLA Copy
Wu, Wentao, et al. “Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising.” Biomedical Optics Express, vol. 15, no. 1, Dec. 2023, p. 114. https://doi.org/10.1364/boe.504550.