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volume 10 issue 5 pages e0128144

Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle

Sebastian O Wendel 1
Sailesh Menon 2
Hamad Alshetaiwi 3
Tej B Shrestha 4
Lauren Chlebanowski 5
Wei-Wen Hsu 6
Stefan H Bossmann 7
Sanjeev Narayanan 2
Deryl L. Troyer 4
Publication typeJournal Article
Publication date2015-05-26
scimago Q1
wos Q2
SJR0.803
CiteScore5.4
Impact factor2.6
ISSN19326203
Multidisciplinary
Abstract
The recent WHO report on antibiotic resistances shows a dramatic increase of microbial resistance against antibiotics. With only a few new antibiotics in the pipeline, a different drug delivery approach is urgently needed. We have obtained evidence demonstrating the effectiveness of a cell based drug delivery system that utilizes the innate immune system as targeting carrier for antibacterial drugs. In this study we show the efficient loading of neutrophil granulocytes with chlorhexidine and the complete killing of E. coli as well as Fusobacterium necrophorum in in-vitro studies. Fusobacterium necrophorum causes hepatic abscesses in cattle fed high grain diets. We also show in a mouse model that this delivery system targets infections of F. necrophorum in the liver and reduces the bacterial burden by an order of magnitude from approximately 2•106 to 1•105.
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GOST |
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GOST Copy
Wendel S. O. et al. Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle // PLoS ONE. 2015. Vol. 10. No. 5. p. e0128144.
GOST all authors (up to 50) Copy
Wendel S. O., Menon S., Alshetaiwi H., Shrestha T. B., Chlebanowski L., Hsu W., Bossmann S. H., Narayanan S., Troyer D. L. Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle // PLoS ONE. 2015. Vol. 10. No. 5. p. e0128144.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1371/journal.pone.0128144
UR - https://doi.org/10.1371/journal.pone.0128144
TI - Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle
T2 - PLoS ONE
AU - Wendel, Sebastian O
AU - Menon, Sailesh
AU - Alshetaiwi, Hamad
AU - Shrestha, Tej B
AU - Chlebanowski, Lauren
AU - Hsu, Wei-Wen
AU - Bossmann, Stefan H
AU - Narayanan, Sanjeev
AU - Troyer, Deryl L.
PY - 2015
DA - 2015/05/26
PB - Public Library of Science (PLoS)
SP - e0128144
IS - 5
VL - 10
PMID - 26011247
SN - 1932-6203
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2015_Wendel,
author = {Sebastian O Wendel and Sailesh Menon and Hamad Alshetaiwi and Tej B Shrestha and Lauren Chlebanowski and Wei-Wen Hsu and Stefan H Bossmann and Sanjeev Narayanan and Deryl L. Troyer},
title = {Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle},
journal = {PLoS ONE},
year = {2015},
volume = {10},
publisher = {Public Library of Science (PLoS)},
month = {may},
url = {https://doi.org/10.1371/journal.pone.0128144},
number = {5},
pages = {e0128144},
doi = {10.1371/journal.pone.0128144}
}
MLA
Cite this
MLA Copy
Wendel, Sebastian O., et al. “Cell Based Drug Delivery: Micrococcus luteus Loaded Neutrophils as Chlorhexidine Delivery Vehicles in a Mouse Model of Liver Abscesses in Cattle.” PLoS ONE, vol. 10, no. 5, May. 2015, p. e0128144. https://doi.org/10.1371/journal.pone.0128144.