Open Access
PLoS ONE, volume 15, issue 11, pages e0242732
Evaluation of immune and chemical precipitation methods for plasma exosome isolation
Штам Т. А.
1
,
Evtushenko Vladimir
2
,
Samsonov Roman
3
,
Kamyshinsky Roman
4
,
Zabegina Lidia
3
,
Verlov Nikolay
1
,
Burdakov Vladimir
1
,
Garaeva Luiza
1
,
Slyusarenko Maria
3
,
Nikiforova Nadezhda
3
,
Malek Anastasia
3
1
Publication type: Journal Article
Publication date: 2020-11-24
Multidisciplinary
Abstract
Exosomes are a type of extracellular vesicles (EVs) secreted by multiple mammalian cell types and involved in intercellular communication. Numerous studies have explored the diagnostic and therapeutic potential of exosomes. The key challenge is the lack of efficient and standard techniques for isolation and downstream analysis of nanovesicles. Conventional isolation methods, such as ultracentrifugation, precipitation, filtration, chromatography, and immune-affinity-based approaches, rely on specific physical properties or on surface biomarkers. However, any of the existing methods has its limitations. Various parameters, such as efficacy, specificity, labor input, cost and scalability, and standardization options, must be considered for the correct choice of appropriate approach. The isolation of exosomes from biological fluids is especially challenged by the complex nature and variability of these liquids. Here, we present a comparison of five protocols for exosome isolation from human plasma: two chemical affinity precipitation methods (lectin-based purification and SubX™ technology), immunoaffinity precipitation, and reference ultracentrifugation-based exosome isolation method in two modifications. An approach for the isolation of exosomes based on the phenomenon of binding and aggregation of these particles via clusters of outer membrane phosphate groups in the presence of SubX™ molecules has been put forward in the present study. The isolated EVs were characterized based upon size, quantity, and protein content.
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Штам Т. А. et al. Evaluation of immune and chemical precipitation methods for plasma exosome isolation // PLoS ONE. 2020. Vol. 15. No. 11. p. e0242732.
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Штам Т. А., Evtushenko V., Samsonov R., Zabrodskaya Y. A., Kamyshinsky R., Zabegina L., Verlov N., Burdakov V., Garaeva L., Slyusarenko M., Nikiforova N., Konevega A. L., Malek A. Evaluation of immune and chemical precipitation methods for plasma exosome isolation // PLoS ONE. 2020. Vol. 15. No. 11. p. e0242732.
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TY - JOUR
DO - 10.1371/journal.pone.0242732
UR - https://doi.org/10.1371%2Fjournal.pone.0242732
TI - Evaluation of immune and chemical precipitation methods for plasma exosome isolation
T2 - PLoS ONE
AU - Штам, Т. А.
AU - Evtushenko, Vladimir
AU - Samsonov, Roman
AU - Zabrodskaya, Yana A.
AU - Kamyshinsky, Roman
AU - Zabegina, Lidia
AU - Verlov, Nikolay
AU - Burdakov, Vladimir
AU - Garaeva, Luiza
AU - Slyusarenko, Maria
AU - Nikiforova, Nadezhda
AU - Konevega, Andrey L.
AU - Malek, Anastasia
PY - 2020
DA - 2020/11/24 00:00:00
PB - Public Library of Science (PLoS)
SP - e0242732
IS - 11
VL - 15
SN - 1932-6203
ER -
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@article{2020_Штам,
author = {Т. А. Штам and Vladimir Evtushenko and Roman Samsonov and Yana A. Zabrodskaya and Roman Kamyshinsky and Lidia Zabegina and Nikolay Verlov and Vladimir Burdakov and Luiza Garaeva and Maria Slyusarenko and Nadezhda Nikiforova and Andrey L. Konevega and Anastasia Malek},
title = {Evaluation of immune and chemical precipitation methods for plasma exosome isolation},
journal = {PLoS ONE},
year = {2020},
volume = {15},
publisher = {Public Library of Science (PLoS)},
month = {nov},
url = {https://doi.org/10.1371%2Fjournal.pone.0242732},
number = {11},
pages = {e0242732},
doi = {10.1371/journal.pone.0242732}
}
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Штам, Т. А., et al. “Evaluation of immune and chemical precipitation methods for plasma exosome isolation.” PLoS ONE, vol. 15, no. 11, Nov. 2020, p. e0242732. https://doi.org/10.1371%2Fjournal.pone.0242732.
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