Open Access
Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study
P Fikar
1
,
Laura Alvarez
1
,
Laura Berne
1
,
Martin Cienciala
1
,
Christopher Kan
1
,
Hynek Kasl
1
,
Mona Luo
1
,
Zuzana Novackova
1
,
Sheyla Ordonez
1
,
Zuzana Sramkova
1
,
Monika Holubova
2
,
Daniel Lysak
2
,
Lyndsay Avery
3
,
Andres A. Caro
4
,
Roslyn N Crowder
5
,
Laura A. Diaz-Martinez
6
,
David W Donley
7
,
Rebecca R. Giorno
8
,
Irene K Guttilla Reed
9
,
Lori L. Hensley
10
,
Kristen C Johnson
11
,
Audrey Y. Kim
12
,
Paul Y. Kim
12
,
Adriana J Lagier
13
,
Jamie Newman
8
,
Elizabeth Padilla-Crespo
14
,
Nathan S Reyna
15
,
Nikolaos Tsotakos
16
,
Noha N. Al-Saadi
10
,
Tayler Appleton
7
,
Ana Arosemena-Pickett
14
,
Braden A. Bell
6
,
Grace Bing
7
,
Bre Bishop
7
,
Christa Forde
12
,
Michael J Foster
8
,
Kassidy Gray
15
,
Bennett L. Hasley
10
,
Kennedy Johnson
15
,
Destiny J. Jones
12
,
Allison C. LaShall
10
,
Kennedy McGuire
15
,
Naomi McNaughton
7
,
Angelina M. Morgan
12
,
Lucas Norris
8
,
Landon A. Ossman
8
,
Paollette A. Rivera-Torres
14
,
Madeline E. Robison
8
,
Kathryn Thibodaux
8
,
Lescia Valmond
12
,
Daniel Georgiev
17
1
Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America
|
2
Department of Hematology and Oncology, University Hospital Pilsen, Pilsen, Czech Republic
|
3
Department of Biology, Saint Michael’s College, Colchester, Vermont, United States of America
|
4
Chemistry Department, Hendrix College, Conway, Arkansas, United States of America
|
7
Department of Biology, Harding University, Searcy, Arkansas, United States of America
|
9
Department of Biology, University of Saint Joseph, West Hartford, Connecticut, United States of America
|
12
Department of Biological Sciences, Grambling State University, Grambling, Louisiana, United States of America
|
13
Department of Biology, College of Social and Natural Sciences, Grand View University, Des Moines, Iowa, United States of America
|
15
Department of Biology, Ouachita Baptist University, Arkadelphia, Arkansas, United States of America
|
17
Department of Research & Development, Sampling Human Inc., Berkeley, California, United States of America,
|
Publication type: Journal Article
Publication date: 2024-12-09
scimago Q1
wos Q2
SJR: 0.803
CiteScore: 5.4
Impact factor: 2.6
ISSN: 19326203
PubMed ID:
39652549
Abstract
Biomedicine today is experiencing a shift towards decentralized data collection, which promises enhanced reproducibility and collaboration across diverse laboratory environments. This inter-laboratory study evaluates the performance of biocytometry, a method utilizing engineered bioparticles for enumerating cells based on their surface antigen patterns. In centralized and aggregated inter-lab studies, biocytometry demonstrated significant statistical power in discriminating numbers of target cells at varying concentrations as low as 1 cell per 100,000 background cells. User skill levels varied from expert to beginner capturing a range of proficiencies. Measurement was performed in a decentralized environment without any instrument cross-calibration or advanced user training outside of a basic instruction manual. The results affirm biocytometry to be a viable solution for immunophenotyping applications demanding sensitivity as well as scalability and reproducibility and paves the way for decentralized analysis of rare cells in heterogeneous samples.
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Fikar P. et al. Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study // PLoS ONE. 2024. Vol. 19. No. 12. p. e0314992.
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Fikar P. et al. Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study // PLoS ONE. 2024. Vol. 19. No. 12. p. e0314992.
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@article{2024_Fikar,
author = {P Fikar and Laura Alvarez and Laura Berne and Martin Cienciala and Christopher Kan and Hynek Kasl and Mona Luo and Zuzana Novackova and Sheyla Ordonez and Zuzana Sramkova and Monika Holubova and Daniel Lysak and Lyndsay Avery and Andres A. Caro and Roslyn N Crowder and Laura A. Diaz-Martinez and David W Donley and Rebecca R. Giorno and Irene K Guttilla Reed and Lori L. Hensley and Kristen C Johnson and Audrey Y. Kim and Paul Y. Kim and Adriana J Lagier and Jamie Newman and Elizabeth Padilla-Crespo and Nathan S Reyna and Nikolaos Tsotakos and Noha N. Al-Saadi and Tayler Appleton and Ana Arosemena-Pickett and Braden A. Bell and Grace Bing and Bre Bishop and Christa Forde and Michael J Foster and Kassidy Gray and Bennett L. Hasley and Kennedy Johnson and Destiny J. Jones and Allison C. LaShall and Kennedy McGuire and Naomi McNaughton and Angelina M. Morgan and Lucas Norris and Landon A. Ossman and Paollette A. Rivera-Torres and Madeline E. Robison and Kathryn Thibodaux and Lescia Valmond and others},
title = {Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study},
journal = {PLoS ONE},
year = {2024},
volume = {19},
publisher = {Public Library of Science (PLoS)},
month = {dec},
url = {https://dx.plos.org/10.1371/journal.pone.0314992},
number = {12},
pages = {e0314992},
doi = {10.1371/journal.pone.0314992}
}
Cite this
MLA
Copy
Fikar, P., et al. “Enhancing reproducibility in single cell research with biocytometry: An inter-laboratory study.” PLoS ONE, vol. 19, no. 12, Dec. 2024, p. e0314992. https://dx.plos.org/10.1371/journal.pone.0314992.