Open Access
PLoS ONE, volume 16, issue 3, pages e0247640
Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma
For Yue Tso
1
,
Salum J. Lidenge
1
,
Lisa K. Poppe
1
,
Phoebe B Peña
1
,
Sara R Privatt
1
,
Sydney J Bennett
1
,
John R Ngowi
2
,
Julius Mwaiselage
3
,
Michael Belshan
1
,
Jacob A. Siedlik
4
,
Morgan A Raine
5
,
Juan B. Ochoa
6
,
Julia Garcia-Diaz
7
,
Bobby Nossaman
7
,
Lyndsey Buckner
7
,
W. Mark Roberts
7
,
Matthew J. Dean
8
,
Augusto C. Ochoa
9
,
John T. West
1
,
Charles C. Wood
1
1
2
Ocean Road Cancer Institute, Dar Es Salaam, Tanzania
|
3
Ocean Road Cancer Institute, Dar es Salaam, Tanzania,
|
6
Department of Surgery, Ochsner Medical Center, New Orleans, LA, United States of America
|
7
Department of Internal Medicine Ochsner Medical Center, New Orleans, LA, United States of America
|
Publication type: Journal Article
Publication date: 2021-03-04
Multidisciplinary
Abstract
Background
Neutralizing-antibody (nAb) is the major focus of most ongoing COVID-19 vaccine trials. However, nAb response against SARS-CoV-2, when present, decays rapidly. Given the myriad roles of antibodies in immune responses, it is possible that antibodies could also mediate protection against SARS-CoV-2 via effector mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), which we sought to explore here.
Methods
Plasma of 3 uninfected controls and 20 subjects exposed to, or recovering from, SARS-CoV-2 infection were collected from U.S. and sub-Saharan Africa. Immunofluorescence assay was used to detect the presence of SARS-CoV-2 specific IgG antibodies in the plasma samples. SARS-CoV-2 specific neutralizing capability of these plasmas was assessed with SARS-CoV-2 spike pseudotyped virus. ADCC activity was assessed with a calcein release assay.
Results
SARS-CoV-2 specific IgG antibodies were detected in all COVID-19 subjects studied. All but three COVID-19 subjects contained nAb at high potency (>80% neutralization). Plasma from 19/20 of COVID-19 subjects also demonstrated strong ADCC activity against SARS-CoV-2 spike glycoprotein, including two individuals without nAb against SARS-CoV-2.
Conclusion
Both neutralizing and non-neutralizing COVID-19 plasmas can mediate ADCC. Our findings argue that evaluation of potential vaccines against SARS-CoV-2 should include investigation of the magnitude and durability of ADCC, in addition to nAb.
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Tso F. Y. et al. Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma // PLoS ONE. 2021. Vol. 16. No. 3. p. e0247640.
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Tso F. Y., Lidenge S. J., Poppe L. K., Peña P. B., Privatt S. R., Bennett S. J., Ngowi J. R., Mwaiselage J., Belshan M., Siedlik J. A., Raine M. A., Ochoa J. B., Garcia-Diaz J., Nossaman B., Buckner L., Roberts W. M., Dean M. J., Ochoa A. C., West J. T., Wood C. C. Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma // PLoS ONE. 2021. Vol. 16. No. 3. p. e0247640.
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TY - JOUR
DO - 10.1371/journal.pone.0247640
UR - https://doi.org/10.1371/journal.pone.0247640
TI - Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma
T2 - PLoS ONE
AU - Tso, For Yue
AU - Lidenge, Salum J.
AU - Poppe, Lisa K.
AU - Peña, Phoebe B
AU - Privatt, Sara R
AU - Bennett, Sydney J
AU - Ngowi, John R
AU - Mwaiselage, Julius
AU - Belshan, Michael
AU - Siedlik, Jacob A.
AU - Raine, Morgan A
AU - Ochoa, Juan B.
AU - Garcia-Diaz, Julia
AU - Nossaman, Bobby
AU - Buckner, Lyndsey
AU - Roberts, W. Mark
AU - Dean, Matthew J.
AU - Ochoa, Augusto C.
AU - West, John T.
AU - Wood, Charles C.
PY - 2021
DA - 2021/03/04
PB - Public Library of Science (PLoS)
SP - e0247640
IS - 3
VL - 16
SN - 1932-6203
ER -
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BibTex (up to 50 authors)
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@article{2021_Tso,
author = {For Yue Tso and Salum J. Lidenge and Lisa K. Poppe and Phoebe B Peña and Sara R Privatt and Sydney J Bennett and John R Ngowi and Julius Mwaiselage and Michael Belshan and Jacob A. Siedlik and Morgan A Raine and Juan B. Ochoa and Julia Garcia-Diaz and Bobby Nossaman and Lyndsey Buckner and W. Mark Roberts and Matthew J. Dean and Augusto C. Ochoa and John T. West and Charles C. Wood},
title = {Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma},
journal = {PLoS ONE},
year = {2021},
volume = {16},
publisher = {Public Library of Science (PLoS)},
month = {mar},
url = {https://doi.org/10.1371/journal.pone.0247640},
number = {3},
pages = {e0247640},
doi = {10.1371/journal.pone.0247640}
}
Cite this
MLA
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Tso, For Yue, et al. “Presence of antibody-dependent cellular cytotoxicity (ADCC) against SARS-CoV-2 in COVID-19 plasma.” PLoS ONE, vol. 16, no. 3, Mar. 2021, p. e0247640. https://doi.org/10.1371/journal.pone.0247640.