Open Access
PLoS ONE, volume 9, issue 10, pages e109263
Colorimetric Aptasensor Using Unmodified Gold Nanoparticles for Homogeneous Multiplex Detection
1
Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, China,
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Publication type: Journal Article
Publication date: 2014-10-03
Multidisciplinary
Abstract
Colorimetric aptasensors using unmodified gold nanoparticles (AuNPs) have attracted much attention because of their low cost, simplicity, and practicality, and they have been developed for various targets in the past several years. However, previous research has focused on developing single-target assays. Here, we report the development of a homogeneous multiplex aptasensor by using more than one class of aptamers to stabilize AuNPs. Using sulfadimethoxine (SDM), kanamycin (KAN) and adenosine (ADE) as example targets, a KAN aptamer (750 nM), an SDM aptamer (250 nM) and an ADE aptamer (500 nM) were mixed at a 1∶1∶1 volume ratio and adsorbed directly onto the surface of unmodified AuNPs by electrostatic interaction. Upon the addition of any of the three targets, the conformation of the corresponding aptamer changed from a random coil structure to a rigid folded structure, which could not adsorb and stabilize AuNPs. The AuNPs aggregated in a specific reaction buffer (20 mM Tris-HCl containing 20 mM NaCl and 5 mM KCl), which led to a color change from red to purple/blue. These results demonstrate that the multiplex colorimetric aptasensor detected three targets simultaneously while maintaining the same sensitivity as a single-target aptasensor for each individual target. The multiplex aptasensor could be extended to other aptamers for various molecular detection events. Due to its simple design, easy operation, fast response, cost effectiveness and lack of need for sophisticated instrumentation, the proposed strategy provides a powerful tool to examine large numbers of samples to screen for a small number of potentially positive samples containing more than one analyte, which can be further validated using sophisticated instruments.
Top-30
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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1 publication, 1.72%
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Citations by publishers
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16
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Elsevier
16 publications, 27.59%
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Multidisciplinary Digital Publishing Institute (MDPI)
8 publications, 13.79%
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American Chemical Society (ACS)
7 publications, 12.07%
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Royal Society of Chemistry (RSC)
7 publications, 12.07%
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Springer Nature
5 publications, 8.62%
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Wiley
4 publications, 6.9%
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SAGE
1 publication, 1.72%
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Taylor & Francis
1 publication, 1.72%
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Oxford University Press
1 publication, 1.72%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 1.72%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Niu S. et al. Colorimetric Aptasensor Using Unmodified Gold Nanoparticles for Homogeneous Multiplex Detection // PLoS ONE. 2014. Vol. 9. No. 10. p. e109263.
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Niu S., Lv Z., Liu J., Bai W., YANG S., Chen A. Colorimetric Aptasensor Using Unmodified Gold Nanoparticles for Homogeneous Multiplex Detection // PLoS ONE. 2014. Vol. 9. No. 10. p. e109263.
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TY - JOUR
DO - 10.1371/journal.pone.0109263
UR - https://doi.org/10.1371/journal.pone.0109263
TI - Colorimetric Aptasensor Using Unmodified Gold Nanoparticles for Homogeneous Multiplex Detection
T2 - PLoS ONE
AU - Niu, Shucao
AU - Lv, Zhenzhen
AU - Liu, Jinchuan
AU - Bai, Wenhui
AU - YANG, SHUMING
AU - Chen, Ailiang
PY - 2014
DA - 2014/10/03 00:00:00
PB - Public Library of Science (PLoS)
SP - e109263
IS - 10
VL - 9
SN - 1932-6203
SN - 1932-6203
ER -
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@article{2014_Niu,
author = {Shucao Niu and Zhenzhen Lv and Jinchuan Liu and Wenhui Bai and SHUMING YANG and Ailiang Chen},
title = {Colorimetric Aptasensor Using Unmodified Gold Nanoparticles for Homogeneous Multiplex Detection},
journal = {PLoS ONE},
year = {2014},
volume = {9},
publisher = {Public Library of Science (PLoS)},
month = {oct},
url = {https://doi.org/10.1371/journal.pone.0109263},
number = {10},
pages = {e109263},
doi = {10.1371/journal.pone.0109263}
}
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Niu, Shucao, et al. “Colorimetric Aptasensor Using Unmodified Gold Nanoparticles for Homogeneous Multiplex Detection.” PLoS ONE, vol. 9, no. 10, Oct. 2014, p. e109263. https://doi.org/10.1371/journal.pone.0109263.