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PLoS ONE, volume 10, issue 8, pages e0134906

Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold

Lomonosova Anna V 1
Ovchinnikova Elena V 1
Kazakov Alexei S 1
DENESYUK Alexander I. 2
Sofin Alexander D 1
Mikhailov Roman V 3
Mirzabekov Tajib A 4
Permyakov Eugene A. 1
Permyakov Sergei E. 1
Publication typeJournal Article
Publication date2015-08-06
Journal: PLoS ONE
Quartile SCImago
Q1
Quartile WOS
Q2
Impact factor3.7
ISSN19326203
Multidisciplinary
Abstract
Due to their remarkably high structural stability, proteins from extremophiles are particularly useful in numerous biological applications. Their utility as alternative protein scaffolds could be especially valuable in small antibody mimetic engineering. These artificial binding proteins occupy a specific niche between antibodies and low molecular weight substances, paving the way for development of innovative approaches in therapeutics, diagnostics, and reagent use. Here, the 50S ribosomal RNA-binding protein L35Ae from the extremophilic archaea Pyrococcus horikoshii has been probed for its potential to serve as a backbone in alternative scaffold engineering. The recombinant wild type L35Ae has a native-like secondary structure, extreme thermal stability (mid-transition temperature of 90°C) and a moderate resistance to the denaturation by guanidine hydrochloride (half-transition at 2.6 M). Chemical crosslinking and dynamic light scattering data revealed that the wild type L35Ae protein has a propensity for multimerization and aggregation correlating with its non-specific binding to a model cell surface of HEK293 cells, as evidenced by flow cytometry. To suppress these negative features, a 10-amino acid mutant (called L35Ae 10X) was designed, which lacks the interaction with HEK293 cells, is less susceptible to aggregation, and maintains native-like secondary structure and thermal stability. However, L35Ae 10X also shows lowered resistance to guanidine hydrochloride (half-transition at 2.0M) and is more prone to oligomerization. This investigation of an extremophile protein’s scaffolding potential demonstrates that lowered resistance to charged chemical denaturants and increased propensity to multimerization may limit the utility of extremophile proteins as alternative scaffolds.

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Lomonosova A. V. et al. Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold // PLoS ONE. 2015. Vol. 10. No. 8. p. e0134906.
GOST all authors (up to 50) Copy
Lomonosova A. V., Ovchinnikova E. V., Kazakov A. S., DENESYUK A. I., Sofin A. D., Mikhailov R. V., Ulitin A. B., Mirzabekov T. A., Permyakov E., Permyakov S. Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold // PLoS ONE. 2015. Vol. 10. No. 8. p. e0134906.
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RIS Copy
TY - JOUR
DO - 10.1371/journal.pone.0134906
UR - https://doi.org/10.1371%2Fjournal.pone.0134906
TI - Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold
T2 - PLoS ONE
AU - Lomonosova, Anna V
AU - Ovchinnikova, Elena V
AU - Kazakov, Alexei S
AU - DENESYUK, Alexander I.
AU - Sofin, Alexander D
AU - Mikhailov, Roman V
AU - Ulitin, Andrei B
AU - Mirzabekov, Tajib A
AU - Permyakov, Eugene A.
AU - Permyakov, Sergei E.
PY - 2015
DA - 2015/08/06 00:00:00
PB - Public Library of Science (PLoS)
SP - e0134906
IS - 8
VL - 10
PMID - 26247602
SN - 1932-6203
ER -
BibTex |
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BibTex Copy
@article{2015_Lomonosova
author = {Anna V Lomonosova and Elena V Ovchinnikova and Alexei S Kazakov and Alexander I. DENESYUK and Alexander D Sofin and Roman V Mikhailov and Andrei B Ulitin and Tajib A Mirzabekov and Eugene A. Permyakov and Sergei E. Permyakov},
title = {Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold},
journal = {PLoS ONE},
year = {2015},
volume = {10},
publisher = {Public Library of Science (PLoS)},
month = {aug},
url = {https://doi.org/10.1371%2Fjournal.pone.0134906},
number = {8},
pages = {e0134906},
doi = {10.1371/journal.pone.0134906}
}
MLA
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MLA Copy
Lomonosova, Anna V., et al. “Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold.” PLoS ONE, vol. 10, no. 8, Aug. 2015, p. e0134906. https://doi.org/10.1371%2Fjournal.pone.0134906.
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