Acta Chromatographica, volume 37, issue 1, pages 87-95

A simple, rapid, and green method for determination of three cholic acids in bio-transformed Jindanfen by HPLC-MS

Zhengming Qian 1, 2
Qi Huang 2
Feng Wang 3
Qinggui Lei 2
Zhuobin He 2
Qilong Wu 3
Meng-hua Wu 4
Jin Gao 4
2
 
Ruyuan Nanling Haoshanhaoshui Cordyceps Co., Ltd., Shaoguan, Guangdong 512000, P. R. China
3
 
Chongqing Kinbear Biotechnology Co., Ltd., Chongqing 400799, P. R. China
Publication typeJournal Article
Publication date2025-02-14
scimago Q3
SJR0.344
CiteScore4.0
Impact factor1.7
ISSN12332356, 20835736
Abstract

A simple, rapid, and green high-performance liquid chromatography-mass spectrometry (HPLC-MS) method was developed for determination of tauroursodeoxycholic acid (TUDCA), taurocholic acid (TCA), and taurochenodeoxycholic acid (TCDCA) in bio-transformed Jindanfen (BTJDF), which is obtained from chicken bile through a bioconversion process. The sample was prepared using water. The HPLC separation was operated on a poroshell 120 EC-C18 column with a 2.0 min gradient elution procedure. Detection was performed on a single quadrupole mass spectrometer in negative mode with selected ion monitoring mode (SIM). This developed HPLC-MS method presented good linearity (r > 0.997) and sensitivity (limit of quantification, 30.0–80.0 pg) for three analytes. The relative standard deviations (RSDs) for precision, repeatability, and stability were all below 3.00%. The matrix effects and average recoveries of three analytes were 91.2–97.9% (RSDs < 1.50%) and 95.4–103% (RSDs < 3.00%), respectively. The average contents of TUDCA, TCA, and TCDCA in ten batches of samples were 33.8, 13.2, and 20.5%, respectively. Finally, the greenness of the developed method was validated by Analytical Eco-Scale and Complex-GAPI. The developed method was proved to be an eco-friendly, effective, and reliable approach for assaying the three cholic acids in BTJDF, which is help to improve the quality evaluation level of the BTJDF industry.

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