USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL

Daniel Dantas FERRARIN 1
O Malafaia 2
Nicolau Gregori CZECZKO 2
Luiz Fernando Kubrusly 2
Marcos Sigwalt 2
Eros Luiz de SOUSA 3
João Carlos Domingues REPKA 3
Pedro Henrique Lambach CARON 3
1
 
Faculdade Evangélica Mackenzie, Brazil; Hospital e Maternidade Angelica Caron, Brazil
2
 
Faculdade Evangélica Mackenzie, Brazil
3
 
Hospital e Maternidade Angelica Caron, Brazil
Publication typeJournal Article
Publication date2024-12-16
scimago Q2
wos Q3
SJR0.405
CiteScore2.3
Impact factor1.8
ISSN01026720, 23176326
Abstract

ABSTRACT BACKGROUND: Nowdays, more relevant applications of perinatal derivatives, such as amniotic membrane (AM), are emerging in our environment as a source of biomaterials for use in different healing processes. The study of anastomosis healing associated with antimetabolic drugs such as 5-fluorouracil (5-FU) is a potential target of AM. AIMS: To evaluate the healing effects of AM in rats treated with 5-FU at a dose of 20 mg/kg on the seventh day of postoperative evolution, regarding the parameters percentage of type I collagen (mature), cell viability, microvascular density and formation of granulation tissue. METHODS: Thirty-two Wistar rats were used, submitted to colotomy and colorraphy, separated into four groups of eight, which received different treatments daily, intraperitoneally, until the day of sacrifice: saline solution (C), 20 mg/kg 5-FU, 20 mg/kg 5-FU and AM. RESULTS: Treatment with 20 mg/kg of 5-FU, on the seventh postoperative day, induced adverse effects on the anastomotic healing process, evidenced by a decrease in the percentage of type I (mature) collagen, cell viability, microvascular density, fibrin-leukocyte scab formation and angiofibroblast proliferation; the use of AM under these conditions induced an improvement in the percentage of type I (mature) collagen. CONCLUSIONS: Treatment with 20 mg/kg of 5-FU on the seventh postoperative day induced adverse effects on the anastomotic healing process, and the use of AM under these conditions induced an improvement in the percentage of type I (mature) collagen.

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FERRARIN D. D. et al. USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL // Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery. 2024. Vol. 37.
GOST all authors (up to 50) Copy
FERRARIN D. D., Malafaia O., CZECZKO N. G., Kubrusly L. F., Sigwalt M., SOUSA E. L. D., REPKA J. C. D., CARON P. H. L. USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL // Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery. 2024. Vol. 37.
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TY - JOUR
DO - 10.1590/0102-6720202400049e1843
UR - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202024000100328&tlng=en
TI - USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL
T2 - Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery
AU - FERRARIN, Daniel Dantas
AU - Malafaia, O
AU - CZECZKO, Nicolau Gregori
AU - Kubrusly, Luiz Fernando
AU - Sigwalt, Marcos
AU - SOUSA, Eros Luiz de
AU - REPKA, João Carlos Domingues
AU - CARON, Pedro Henrique Lambach
PY - 2024
DA - 2024/12/16
PB - Colegio Brasileiro de Cirurgia Digestiva
VL - 37
PMID - 39699379
SN - 0102-6720
SN - 2317-6326
ER -
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@article{2024_FERRARIN,
author = {Daniel Dantas FERRARIN and O Malafaia and Nicolau Gregori CZECZKO and Luiz Fernando Kubrusly and Marcos Sigwalt and Eros Luiz de SOUSA and João Carlos Domingues REPKA and Pedro Henrique Lambach CARON},
title = {USE OF DECELLULARIZED HUMAN AMNIOTIC MEMBRANE IN INTESTINAL ANASTOMOSES: A STUDY IN RATS TREATED WITH 5-FLUOROURACIL},
journal = {Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery},
year = {2024},
volume = {37},
publisher = {Colegio Brasileiro de Cirurgia Digestiva},
month = {dec},
url = {http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-67202024000100328&tlng=en},
doi = {10.1590/0102-6720202400049e1843}
}