PROCESSING AND RESEARCH OF EFFECTIVENESS OF NEW BIOPREPARATES BASED ON RHODOCOCCUS SP. H33, GUMATES AND LIGNOSULFONATES FOR THE CLEANING OF NEFTEZAINOUSLY CONTAMINATED SOILS

Development of preparations for increasing the efficiency of carbon deposition by woody plants in the conditions of technogenically contaminated lands is an urgent task and is divided into 2 stages. The first stage is related to the remediation of lands polluted with hydrocarbons in the process of oil production and refining. The second stage is aimed at obtaining biopreparations to accelerate carbon deposition by biosystems (woody and herbaceous plants).In this work, the effectiveness of a biopreparation based on a new strain of bacteria-oil-destructors Rhodococcus sp. H33 in combination with humates and lignosulfonates for cleaning of oil-contaminated lands was studied.It is shown that the most effective of several strains of oil destructors isolated from oil-contaminated soils is Rhodococcussp. H33, which was used as the basis of biopreparation for cleaning of lands contaminated with oil and oil products.It was found that at 8 % level of oil pollution microorganisms of the biopreparation based on strain H33 together with additives are capable of its greater destruction (the degree of biodegradation in the variant with lignosulfonates reaches 47.4 % for 6 weeks of experiment) than components separately (introduction of strain H33 without additives leads to destruction of 20 % of oil, humic preparations — does not exceed 10 %), comparison preparation «Lenoil» — 15–20 %. Based on the values of the number of heterotrophic, as well as hydrocarbon- oxidizing microorganisms, as well as the results of reducing the concentration of oil hydrocarbons, it is shown that the following composition of the complex biotechnological preparation-oil-destructor is optimal: biopreparation based on bacterial strain H33 (titer 2·109 CFU/ ml) — NPK — complex fertilizer (nitrogen, phosphorus, potassium) 0.25 g/kg soil — 0.02 % wt. sodium lignosulfonate.