Open Access
Open access
Publishing House ABV Press
Advances in molecular oncology
,  volume 9 ,  issue 2 ,  pages 58-65

Hypoxia-related proteins VEGF A and CA IX and resistance of soft tissue sarcoma cells to chemotherapy: ex vivo pilot study

Alexander M. Scherbakov 1
, 
T I Fetisov 1
, 
Dmytro Sorokin 1
, 
V Yu Zinovieva 1
, 
Natalia I Moiseeva 1
, 
L A Laletina 1
, 
Evgeny M Kirilin 2
, 
A E Manikaylo 1
, 
L Ya Fomina 1
, 
L V Mekheda 1
, 
B. Bokhyan 1
, 
Ekaterina A. Lesovaya 1
, 
L S Trukhanova 1
, 
T G Gorkova 1
, 
E E Antoshina 1
, 
O. V. Morozova 1
, 
Kirill I. Kirsanov 3
, 
Marianna G. Yakubovskaya 1
1
 
2
 
3
 
Publication type: Journal Article
Publication date: 2022-06-25
Publishing House ABV Press
scimago Q4
SJR: 0.145
CiteScore: 0.5
Impact factor: —
ISSN: 2313805X, 24133787
Cancer Research
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Genetics (clinical)
Biochemistry (medical)
Abstract

Introduction. The identification of predictive factors is a cornerstone task of modern oncology. The development of new targeted drugs determines the need for prediction of chemosensitivity of each patient to the prescribed therapy, in this regard, the search for biomarkers of predictive response to therapy is actively conducted.

The study objective to investigate the relationship between tumor cell resistance and the expression levels of CA IX (carbonic anhydrase IX) and VEGF A (vascular endothelial growth factor А) in patient-derived cultures of soft tissue sarcomas.

Materials and methods: ex vivo soft tissue sarcoma cell culture, resazurin test, immunoblotting.

Results. We obtained 46 ex vivo samples of soft tissue sarcoma cultures for which chemosensitivity to doxorubicin, ifosfamide, docetaxel, gemcitabine, and their combinations was assessed by the resazurin cytotoxicity test. We analyzed the relationship between the expression of hypoxic proteins VEGF A and CA IX and the resistance to drugs. A correlation between the CA IX expression in hypoxia and cell resistance to ifosfamide and its combination with doxorubicin was found. Soft tissue sarcomas with high VEGF A index were resistant to doxorubicin, docetaxel, and its combination with gemcitabine (p <0.05).

Conclusion. The data obtained on patient-derived cultures indicate the relationship between hypoxic signaling and resistance of soft tissue sarcomas to chemotherapeutics.

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Scherbakov A. M. et al. Hypoxia-related proteins VEGF A and CA IX and resistance of soft tissue sarcoma cells to chemotherapy: ex vivo pilot study // Advances in molecular oncology. 2022. Vol. 9. No. 2. pp. 58-65.
GOST all authors (up to 50) Copy
Scherbakov A. M., Fetisov T. I., Sorokin D., Zinovieva V. Yu., Moiseeva N. I., Laletina L. A., Kirilin E. M., Manikaylo A. E., Fomina L. Ya., Mekheda L. V., Bokhyan B., Lesovaya E. A., Trukhanova L. S., Gorkova T. G., Antoshina E. E., Morozova O. V., Kirsanov K. I., Yakubovskaya M. G. Hypoxia-related proteins VEGF A and CA IX and resistance of soft tissue sarcoma cells to chemotherapy: ex vivo pilot study // Advances in molecular oncology. 2022. Vol. 9. No. 2. pp. 58-65.
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RIS Copy
TY - JOUR
DO - 10.17650/2313-805X-2022-9-2-58-65
UR - https://umo.abvpress.ru/jour/article/view/439
TI - Hypoxia-related proteins VEGF A and CA IX and resistance of soft tissue sarcoma cells to chemotherapy: ex vivo pilot study
T2 - Advances in molecular oncology
AU - Scherbakov, Alexander M.
AU - Fetisov, T I
AU - Sorokin, Dmytro
AU - Zinovieva, V Yu
AU - Moiseeva, Natalia I
AU - Laletina, L A
AU - Kirilin, Evgeny M
AU - Manikaylo, A E
AU - Fomina, L Ya
AU - Mekheda, L V
AU - Bokhyan, B.
AU - Lesovaya, Ekaterina A.
AU - Trukhanova, L S
AU - Gorkova, T G
AU - Antoshina, E E
AU - Morozova, O. V.
AU - Kirsanov, Kirill I.
AU - Yakubovskaya, Marianna G.
PY - 2022
DA - 2022/06/25
PB - Publishing House ABV Press
SP - 58-65
IS - 2
VL - 9
SN - 2313-805X
SN - 2413-3787
ER -
BibTex |
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@article{2022_Scherbakov,
author = {Alexander M. Scherbakov and T I Fetisov and Dmytro Sorokin and V Yu Zinovieva and Natalia I Moiseeva and L A Laletina and Evgeny M Kirilin and A E Manikaylo and L Ya Fomina and L V Mekheda and B. Bokhyan and Ekaterina A. Lesovaya and L S Trukhanova and T G Gorkova and E E Antoshina and O. V. Morozova and Kirill I. Kirsanov and Marianna G. Yakubovskaya},
title = {Hypoxia-related proteins VEGF A and CA IX and resistance of soft tissue sarcoma cells to chemotherapy: ex vivo pilot study},
journal = {Advances in molecular oncology},
year = {2022},
volume = {9},
publisher = {Publishing House ABV Press},
month = {jun},
url = {https://umo.abvpress.ru/jour/article/view/439},
number = {2},
pages = {58--65},
doi = {10.17650/2313-805X-2022-9-2-58-65}
}
MLA
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Scherbakov, Alexander M., et al. “Hypoxia-related proteins VEGF A and CA IX and resistance of soft tissue sarcoma cells to chemotherapy: ex vivo pilot study.” Advances in molecular oncology, vol. 9, no. 2, Jun. 2022, pp. 58-65. https://umo.abvpress.ru/jour/article/view/439.