Open Access
Open access
Tomsk Cancer Research Institute
Siberian Journal of Oncology
,  volume 21 ,  issue 3 ,  pages 50-60

In vitro screening of effectiveness and antiproliferative effects of potential ddit4 inhibitors for breast cancer cell lines

Ekaterina M. Zhidkova 1
, 
D D Grigoreva 1
, 
E S Lylova 1
, 
Varvara Maksimova 1
, 
G R Sagitova 2
, 
G I Khayrieva 2
, 
E S Trapeznikova 2
, 
Kirill I. Kirsanov 3
, 
Marianna G. Yakubovskaya 1
, 
Ekaterina A. Lesovaya 4
1
 
2
 
3
 
4
 
Publication type: Journal Article
Publication date: 2022-06-29
Tomsk Cancer Research Institute
scimago Q4
SJR: 0.134
CiteScore: 0.6
Impact factor: —
ISSN: 18144861, 23123168
Cancer Research
Oncology
Abstract

Objective: screening of previously selected DDIT4 inhibitors by their ability to suppress basal and glucocorticoid-induced expression of this gene in breast cancer (BC) cells, as well as evaluation of antiproliferative and cytotoxic effects of the studied drug combinations the antiproliferative and proapoptotic effects of studied drug combinations. Material and Methods. Breast cancer cells of the luminal, HER2- positive and triple negative subtypes were used. The effects of drugs (rapamycin, wortmannin, LY-294002, apigenin, resveratrol, curcumin, CGP-60474, and emetine) on the basal and glucocorticoid-induced levels of expression of the DDIT4 gene and its protein product were evaluated by qPCR and Western blotting assays. Results. Emetine, rapamycin, wortmannin, LY-294002 and CGP-60474 demonstrated DDIT4-inhibition activity. Glucocorticoid dexamethasone showed cytotoxic effects and antiproliferative activity in combination with emetine, CGP-60474 (C protein kinase inhibitor), resveratrol and curcumin. Conclusion. Novel inhibitors of DDIT4 in breast cancer model cells in vitro were found. Emetine and CGP-60474 are the most promising drugs for further research.

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Zhidkova E. M. et al. In vitro screening of effectiveness and antiproliferative effects of potential ddit4 inhibitors for breast cancer cell lines // Siberian Journal of Oncology. 2022. Vol. 21. No. 3. pp. 50-60.
GOST all authors (up to 50) Copy
Zhidkova E. M., Grigoreva D. D., Lylova E. S., Maksimova V., Sagitova G. R., Khayrieva G. I., Trapeznikova E. S., Kirsanov K. I., Yakubovskaya M. G., Lesovaya E. A. In vitro screening of effectiveness and antiproliferative effects of potential ddit4 inhibitors for breast cancer cell lines // Siberian Journal of Oncology. 2022. Vol. 21. No. 3. pp. 50-60.
RIS |
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RIS Copy
TY - JOUR
DO - 10.21294/1814-4861-2022-21-3-50-60
UR - https://doi.org/10.21294/1814-4861-2022-21-3-50-60
TI - In vitro screening of effectiveness and antiproliferative effects of potential ddit4 inhibitors for breast cancer cell lines
T2 - Siberian Journal of Oncology
AU - Zhidkova, Ekaterina M.
AU - Grigoreva, D D
AU - Lylova, E S
AU - Maksimova, Varvara
AU - Sagitova, G R
AU - Khayrieva, G I
AU - Trapeznikova, E S
AU - Kirsanov, Kirill I.
AU - Yakubovskaya, Marianna G.
AU - Lesovaya, Ekaterina A.
PY - 2022
DA - 2022/06/29
PB - Tomsk Cancer Research Institute
SP - 50-60
IS - 3
VL - 21
SN - 1814-4861
SN - 2312-3168
ER -
BibTex |
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BibTex (up to 50 authors) Copy
@article{2022_Zhidkova,
author = {Ekaterina M. Zhidkova and D D Grigoreva and E S Lylova and Varvara Maksimova and G R Sagitova and G I Khayrieva and E S Trapeznikova and Kirill I. Kirsanov and Marianna G. Yakubovskaya and Ekaterina A. Lesovaya},
title = {In vitro screening of effectiveness and antiproliferative effects of potential ddit4 inhibitors for breast cancer cell lines},
journal = {Siberian Journal of Oncology},
year = {2022},
volume = {21},
publisher = {Tomsk Cancer Research Institute},
month = {jun},
url = {https://doi.org/10.21294/1814-4861-2022-21-3-50-60},
number = {3},
pages = {50--60},
doi = {10.21294/1814-4861-2022-21-3-50-60}
}
MLA
Cite this
MLA Copy
Zhidkova, Ekaterina M., et al. “In vitro screening of effectiveness and antiproliferative effects of potential ddit4 inhibitors for breast cancer cell lines.” Siberian Journal of Oncology, vol. 21, no. 3, Jun. 2022, pp. 50-60. https://doi.org/10.21294/1814-4861-2022-21-3-50-60.