PCR past, present and future

Hanliang Zhu ¹

Haoqing Zhang ¹

Ying Xu ¹

Soňa Laššáková ²

Marie Korabečná ²

Pavel Neužil ^{1, 3, 4}

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Department of Microsystem Engineering, School of Mechanical Engineering, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, PR China |

Institute of Biology & Medical Genetics, First Faculty of Medicine, Charles University & General University Hospital in Prague, Purkyně Institute, Albertov 4, Praha 2 128 00, Czech Republic |

Brno University of Technology, Central European Institute of Technology, Purkyňova 123, 612 00 Brno, Czech Republic

⁴

Brno University of Technology, Faculty of Electrical Engineering and Communications, Technická 3058/10, 616 00 Brno, Czech Republic |

Publication type: Journal Article

Publication date: 2020-08-20

Taylor & Francis

Journal: BioTechniques

scimago Q2

SJR: 0.557

CiteScore: 4.4

Impact factor: 2.2

ISSN: 07366205, 19409818

DOI: 10.2144/btn-2020-0057

Copy DOI

PubMed ID: 32815744

General Biochemistry, Genetics and Molecular Biology

Biotechnology

Abstract

PCR has become one of the most valuable techniques currently used in bioscience, diagnostics and forensic science. Here we review the history of PCR development and the technologies that have evolved from the original PCR method. Currently, there are two main areas of PCR utilization in bioscience: high-throughput PCR systems and microfluidics-based PCR devices for point-of-care (POC) applications. We also discuss the commercialization of these techniques and conclude with a look into their modifications and use in innovative areas of biomedicine. For example, real-time reverse transcription PCR is the gold standard for SARS-CoV-2 diagnoses. It could also be used for POC applications, being a key component of the sample-to-answer system.

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