Open Access
Quantitative RT-PCR: pitfalls and potential.
Тип публикации: Journal Article
Дата публикации: 2018-08-22
scimago Q3
wos Q3
white level БС2
SJR: 0.435
CiteScore: 3.3
Impact factor: 2.5
ISSN: 07366205, 19409818
PubMed ID:
9894600
General Biochemistry, Genetics and Molecular Biology
Biotechnology
Краткое описание
Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR involves correction for experimental variations in individual RT and PCR efficiencies. This review addresses the mathematics of RT-PCR, choice of RNA standards (internal vs. external) and quantification strategies (competitive, noncompetitive and kinetic [real-time] amplification). Finally, the discussion turns to practical considerations in experimental design. It is hoped that this review will be appropriate for those undertaking these experiments for the first time or wishing to improve (or validate) a technique in what is frequently a confusing and contradictory field.
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Метрики
768
Всего цитирований:
768
Цитирований c 2025:
28
(3.64%)
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MLA
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ГОСТ
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Freeman W. M., Walker S. J., Vrana K. E. Quantitative RT-PCR: pitfalls and potential. // BioTechniques. 2018. Vol. 26. No. 1. pp. 112-125.
ГОСТ со всеми авторами (до 50)
Скопировать
Freeman W. M., Walker S. J., Vrana K. E. Quantitative RT-PCR: pitfalls and potential. // BioTechniques. 2018. Vol. 26. No. 1. pp. 112-125.
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RIS
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TY - JOUR
DO - 10.2144/99261rv01
UR - https://doi.org/10.2144/99261rv01
TI - Quantitative RT-PCR: pitfalls and potential.
T2 - BioTechniques
AU - Freeman, Willard M.
AU - Walker, Stephen J.
AU - Vrana, Kent E.
PY - 2018
DA - 2018/08/22
PB - Taylor & Francis
SP - 112-125
IS - 1
VL - 26
PMID - 9894600
SN - 0736-6205
SN - 1940-9818
ER -
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BibTex (до 50 авторов)
Скопировать
@article{2018_Freeman,
author = {Willard M. Freeman and Stephen J. Walker and Kent E. Vrana},
title = {Quantitative RT-PCR: pitfalls and potential.},
journal = {BioTechniques},
year = {2018},
volume = {26},
publisher = {Taylor & Francis},
month = {aug},
url = {https://doi.org/10.2144/99261rv01},
number = {1},
pages = {112--125},
doi = {10.2144/99261rv01}
}
Цитировать
MLA
Скопировать
Freeman, Willard M., et al. “Quantitative RT-PCR: pitfalls and potential..” BioTechniques, vol. 26, no. 1, Aug. 2018, pp. 112-125. https://doi.org/10.2144/99261rv01.
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