The identification of the Streptomyces sp. VKM Ac-2618D strain had been carried out and its morphological and physiological features have been studied in relation to the immunosuppressant tacrolimus production. The phenotypic variability of the strain was analyzed and the dissociant with a high level of tacrolimus production was identified. Based on a comprehensive study of morphological, physiological and chemotaxonomic properties, as well as phylogenetic analysis, the strain was named Streptomyces tsukubensis VKM Ac-2618D. The strain genome contains the full version of the tacrolimus biosynthetic gene cluster. The advantages of fed-batch cultivation mode for tacrolimus biosynthesis were shown. The results obtained expand the understanding of the peculiarities of polyketide biosynthesis and can be used in the development of a tacrolimus production technology. Key words: Streptomyces tsukubensis, tacrolimus, FK-506, dissociant, genome, biosynthesis The research was carried out within the framework of the state assignment АААА-А16-116062110077-6.

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Fokina V. V. et al. Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus // Biotekhnologiya. 2021. Vol. 37. No. 1. pp. 26-36.

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Fokina V. V., Tarlachkov S. V., Machulin A., Shutov A. A., Donova M. V. Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus // Biotekhnologiya. 2021. Vol. 37. No. 1. pp. 26-36.

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TY - JOUR

DO - 10.21519/0234-2758-2021-37-1-26-36

UR - https://doi.org/10.21519/0234-2758-2021-37-1-26-36

TI - Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus

T2 - Biotekhnologiya

AU - Fokina, V V

AU - Tarlachkov, S V

AU - Machulin, A.V.

AU - Shutov, A A

AU - Donova, M V

PY - 2021

DA - 2021/03/10

PB - State Research Institute for Genetics and Selection of Industrial Microorganisms

SP - 26-36

IS - 1

VL - 37

SN - 0234-2758

SN - 2500-2341

ER -

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@article{2021_Fokina,

author = {V V Fokina and S V Tarlachkov and A.V. Machulin and A A Shutov and M V Donova},

title = {Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus},

journal = {Biotekhnologiya},

year = {2021},

volume = {37},

publisher = {State Research Institute for Genetics and Selection of Industrial Microorganisms},

month = {mar},

url = {https://doi.org/10.21519/0234-2758-2021-37-1-26-36},

number = {1},

pages = {26--36},

doi = {10.21519/0234-2758-2021-37-1-26-36}

}

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Fokina, V. V., et al. “Streptomyces tsukubensis VKM Aс-2618D - an Effective Producer of Tacrolimus.” Biotekhnologiya, vol. 37, no. 1, Mar. 2021, pp. 26-36. https://doi.org/10.21519/0234-2758-2021-37-1-26-36.