Open Access
Open access
Walter de Gruyter
Acta Veterinaria
,  volume 73 ,  issue 1 ,  pages 13-21

Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)

Publication type: Journal Article
Publication date: 2023-03-01
Walter de Gruyter
scimago Q3
wos Q3
SJR: 0.250
CiteScore: 1.4
Impact factor: 0.8
ISSN: 05678315, 18207448
General Veterinary
Abstract

Viral individual genes functions and their role in the interaction with the host cells remain the main area in the study of African swine fever virus (ASFV) biology. The extreme heterogeneity of the ASFV makes it difficult to develop vaccines against this pathogen. In this work, we generated the ASFV deletion mutant virus Volgograd/D(1L-5-6L) with the six genes deletion in multigenic family 110 (MGF110) (1L-5-6L) and studied its characteristics in vitro. The homologous recombination method was used for the deletion in ASFV parental strain Volgograd/14с. A series of six passages was carried out in the COS-1 cell culture using the limiting dilution method. The recombinant strain Volgograd/D(1L-5-6L) MGF110 was selected by the plaque formation method. Performed study of viral replication showed no changes in viral growth kinetics in comparison with the parental strain. The ASFV Volgograd/D(1L-5-6L) MGF110 is a great tool available to test the importance of MGF110 for virus virulence and vaccine development.

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GOST Copy
Nefedeva M. V. et al. Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II) // Acta Veterinaria. 2023. Vol. 73. No. 1. pp. 13-21.
GOST all authors (up to 50) Copy
Nefedeva M. V., Malogolovkin A. S., Titov I. A. Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II) // Acta Veterinaria. 2023. Vol. 73. No. 1. pp. 13-21.
RIS |
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RIS Copy
TY - JOUR
DO - 10.2478/acve-2023-0002
UR - https://doi.org/10.2478/acve-2023-0002
TI - Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)
T2 - Acta Veterinaria
AU - Nefedeva, Mariia V.
AU - Malogolovkin, Alexander S
AU - Titov, Ilya A
PY - 2023
DA - 2023/03/01
PB - Walter de Gruyter
SP - 13-21
IS - 1
VL - 73
SN - 0567-8315
SN - 1820-7448
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2023_Nefedeva,
author = {Mariia V. Nefedeva and Alexander S Malogolovkin and Ilya A Titov},
title = {Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II)},
journal = {Acta Veterinaria},
year = {2023},
volume = {73},
publisher = {Walter de Gruyter},
month = {mar},
url = {https://doi.org/10.2478/acve-2023-0002},
number = {1},
pages = {13--21},
doi = {10.2478/acve-2023-0002}
}
MLA
Cite this
MLA Copy
Nefedeva, Mariia V., et al. “Design of a Replicative-Competent MGF110 (1L-5-6L) Deleted African Swine Fever Virus (Genotype II).” Acta Veterinaria, vol. 73, no. 1, Mar. 2023, pp. 13-21. https://doi.org/10.2478/acve-2023-0002.