Open Access
Open access
Walter de Gruyter
Journal of Veterinary Research (Poland)
,  volume 66 ,  issue 4 ,  pages 497-510

Phylogenetic analysis of small ruminant lentiviruses originating from naturally infected sheep and goats from Poland based on the long terminal repeat sequences

Monika Olech 1, 2
, 
Jacek Kuźmak 2
, 
Anna Kycko 1
, 
Andrzej Junkuszew 3
1
 
Department of Pathology , Puławy , Poland | 
2
 
Department of Biochemistry, National Veterinary Research Institute , 24-100 Puławy , Poland | 
3
 
Publication type: Journal Article
Publication date: 2022-11-15
Walter de Gruyter
scimago Q2
wos Q2
SJR: 0.472
CiteScore: 2.6
Impact factor: 1.5
ISSN: 24507393, 24508608
General Veterinary
Abstract
Introduction

Previous gag and env sequence studies placed Polish small ruminant lentiviruses (SRLVs) isolated from sheep and goats in subtypes B1, B2, A1, A5, A12, A13, A16–A18, A23, A24 and A27. This study extended the genetic/phylogenetic analysis of previously identified Polish SRLV strains by contributing long terminal repeat (LTR) sequences.
Material and Methods

A total of 112 samples were analysed. Phylogenetic analyses were carried out on the LTR fragment using the neighbour-joining, maximum likelihood, and unweighted pair group method with arithmetic mean methods.
Results

Polish caprine and ovine LTR sequences clustered within group A and grouped in at least 10 clusters (subtypes A1, A5, A12, A13, A16–A18, A23, A24 and A27). Most of the Polish strains (78%) belonged to the same subtype by the indication of the gag, env and LTR genomic regions. Discrepancies in affiliation depending on the particular sequence were observed in 24 (21%) strains, most of which came from mixed-species flocks where more than one SRLV genotype circulated. Sequences of the LTR reflected subtype-specific patterns. Several subtype-specific markers were identified, e.g. a unique substitution of T to A in the fifth position of the TATA box in A17, A27, A20 and B3.
Conclusion

This study provides valuable insights into the genetic diversity of SRLV field strains in Poland, their phylogenetic relationships and their position in the recently established SRLV classification. Our results confirmed the existence of the ten subtypes listed and the readier emergence of new SRLV variants in mixed-species flocks.

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GOST Copy
Olech M. et al. Phylogenetic analysis of small ruminant lentiviruses originating from naturally infected sheep and goats from Poland based on the long terminal repeat sequences // Journal of Veterinary Research (Poland). 2022. Vol. 66. No. 4. pp. 497-510.
GOST all authors (up to 50) Copy
Olech M., Kuźmak J., Kycko A., Junkuszew A. Phylogenetic analysis of small ruminant lentiviruses originating from naturally infected sheep and goats from Poland based on the long terminal repeat sequences // Journal of Veterinary Research (Poland). 2022. Vol. 66. No. 4. pp. 497-510.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.2478/jvetres-2022-0064
UR - https://doi.org/10.2478/jvetres-2022-0064
TI - Phylogenetic analysis of small ruminant lentiviruses originating from naturally infected sheep and goats from Poland based on the long terminal repeat sequences
T2 - Journal of Veterinary Research (Poland)
AU - Olech, Monika
AU - Kuźmak, Jacek
AU - Kycko, Anna
AU - Junkuszew, Andrzej
PY - 2022
DA - 2022/11/15
PB - Walter de Gruyter
SP - 497-510
IS - 4
VL - 66
PMID - 36846042
SN - 2450-7393
SN - 2450-8608
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2022_Olech,
author = {Monika Olech and Jacek Kuźmak and Anna Kycko and Andrzej Junkuszew},
title = {Phylogenetic analysis of small ruminant lentiviruses originating from naturally infected sheep and goats from Poland based on the long terminal repeat sequences},
journal = {Journal of Veterinary Research (Poland)},
year = {2022},
volume = {66},
publisher = {Walter de Gruyter},
month = {nov},
url = {https://doi.org/10.2478/jvetres-2022-0064},
number = {4},
pages = {497--510},
doi = {10.2478/jvetres-2022-0064}
}
MLA
Cite this
MLA Copy
Olech, Monika, et al. “Phylogenetic analysis of small ruminant lentiviruses originating from naturally infected sheep and goats from Poland based on the long terminal repeat sequences.” Journal of Veterinary Research (Poland), vol. 66, no. 4, Nov. 2022, pp. 497-510. https://doi.org/10.2478/jvetres-2022-0064.