Recombinant alpha-amino ester acid hydrolase from Xanthomonas rubrilineans VKPM B-9915 is a highly efficient biocatalyst of cephalexin synthesis
A V Sklyarenko
1
,
O.V. Berezina
1
,
D E Satarova
1
,
V.V. Fedorchuk
2, 3
,
E A Fedorchuk
2, 3
,
S. S. Savin
3, 4
,
S V Yarotsky
1
,
V I Tishkov
2, 3, 4
Publication type: Journal Article
Publication date: 2014-03-01
scimago Q4
wos Q4
SJR: 0.153
CiteScore: 1.1
Impact factor: 0.5
ISSN: 00271314, 19350260
General Chemistry
Abstract
Recombinant, as well as native alpha-amino acid ester hydrolase from Xanthomonas rubrilineans VKPM B-9915 (XrAEH, EC 3.1.1.43), was tested for synthesis of amino-beta-lactam antibiotic cephalexin. It was shown that the recombinant enzyme r-XrAEH produced by Escherichia coli VKPM B-11246 is more efficient in comparison with the native enzyme wt-XrAEH prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. When r-XrAEH was used as a biocatalyst, addition of ethylene glycol (33 vol %) to the reaction medium improved the yield from 70 to 95%. During synthesis of cephalexin under optimal conditions in the case of the native enzyme wt-XrAEH the cephalexin yield was 85%, in contrast to r-XrAEH where it was 95%. Furthermore, unlike native wt-XrAEH enzymes, preparations of recombinant r-XrAEH do not possess beta-lactamase side activity.
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Sklyarenko A. V. et al. Recombinant alpha-amino ester acid hydrolase from Xanthomonas rubrilineans VKPM B-9915 is a highly efficient biocatalyst of cephalexin synthesis // Moscow University Chemistry Bulletin. 2014. Vol. 69. No. 2. pp. 62-67.
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Sklyarenko A. V., Berezina O., Satarova D. E., Fedorchuk V., Fedorchuk E. A., Savin S. S., Yarotsky S. V., Tishkov V. I. Recombinant alpha-amino ester acid hydrolase from Xanthomonas rubrilineans VKPM B-9915 is a highly efficient biocatalyst of cephalexin synthesis // Moscow University Chemistry Bulletin. 2014. Vol. 69. No. 2. pp. 62-67.
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TY - JOUR
DO - 10.3103/s0027131414020084
UR - https://doi.org/10.3103/s0027131414020084
TI - Recombinant alpha-amino ester acid hydrolase from Xanthomonas rubrilineans VKPM B-9915 is a highly efficient biocatalyst of cephalexin synthesis
T2 - Moscow University Chemistry Bulletin
AU - Sklyarenko, A V
AU - Berezina, O.V.
AU - Satarova, D E
AU - Fedorchuk, V.V.
AU - Fedorchuk, E A
AU - Savin, S. S.
AU - Yarotsky, S V
AU - Tishkov, V I
PY - 2014
DA - 2014/03/01
PB - Pleiades Publishing
SP - 62-67
IS - 2
VL - 69
SN - 0027-1314
SN - 1935-0260
ER -
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BibTex (up to 50 authors)
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@article{2014_Sklyarenko,
author = {A V Sklyarenko and O.V. Berezina and D E Satarova and V.V. Fedorchuk and E A Fedorchuk and S. S. Savin and S V Yarotsky and V I Tishkov},
title = {Recombinant alpha-amino ester acid hydrolase from Xanthomonas rubrilineans VKPM B-9915 is a highly efficient biocatalyst of cephalexin synthesis},
journal = {Moscow University Chemistry Bulletin},
year = {2014},
volume = {69},
publisher = {Pleiades Publishing},
month = {mar},
url = {https://doi.org/10.3103/s0027131414020084},
number = {2},
pages = {62--67},
doi = {10.3103/s0027131414020084}
}
Cite this
MLA
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Sklyarenko, A. V., et al. “Recombinant alpha-amino ester acid hydrolase from Xanthomonas rubrilineans VKPM B-9915 is a highly efficient biocatalyst of cephalexin synthesis.” Moscow University Chemistry Bulletin, vol. 69, no. 2, Mar. 2014, pp. 62-67. https://doi.org/10.3103/s0027131414020084.