Nuclei deformation in HaCaT keratinocytes cultivated on aligned fibrous substrates
Publication type: Journal Article
Publication date: 2017-04-01
scimago Q4
SJR: 0.161
CiteScore: 1.1
Impact factor: —
ISSN: 00963925, 1934791X
General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences
General Environmental Science
Abstract
Substrate topography influences cell shape, direction and rate of migration, nucleus shape, and gene expression levels. This influence is commonly studied using substrates with predefined surface structure and chemical composition. In the current work, we studied the state of HaCaT keratinocyte nuclei and actin cytoskeleton on poly(ε-caprolactone) scaffolds obtained by electrospinning. Two types of fibrous scaffolds were prepared and characterized. In the random scaffolds, the fibers were arranged in a nonsystematic fashion; however, most of the fibers had the same direction in the aligned scaffolds. When cultured on the aligned scaffolds, HaCaT cells exhibited oriented actin filaments and had more elongated nuclei.
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Pavlova E. R. et al. Nuclei deformation in HaCaT keratinocytes cultivated on aligned fibrous substrates // Moscow University Biological Sciences Bulletin. 2017. Vol. 72. No. 2. pp. 85-90.
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Pavlova E. R., Bagrov D. V., Khramova Yu. V., Klinov D. V., Shaitan K. V. Nuclei deformation in HaCaT keratinocytes cultivated on aligned fibrous substrates // Moscow University Biological Sciences Bulletin. 2017. Vol. 72. No. 2. pp. 85-90.
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RIS
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TY - JOUR
DO - 10.3103/S0096392517020043
UR - https://doi.org/10.3103/S0096392517020043
TI - Nuclei deformation in HaCaT keratinocytes cultivated on aligned fibrous substrates
T2 - Moscow University Biological Sciences Bulletin
AU - Pavlova, E R
AU - Bagrov, D V
AU - Khramova, Yu V
AU - Klinov, D. V.
AU - Shaitan, K V
PY - 2017
DA - 2017/04/01
PB - Pleiades Publishing
SP - 85-90
IS - 2
VL - 72
SN - 0096-3925
SN - 1934-791X
ER -
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BibTex (up to 50 authors)
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@article{2017_Pavlova,
author = {E R Pavlova and D V Bagrov and Yu V Khramova and D. V. Klinov and K V Shaitan},
title = {Nuclei deformation in HaCaT keratinocytes cultivated on aligned fibrous substrates},
journal = {Moscow University Biological Sciences Bulletin},
year = {2017},
volume = {72},
publisher = {Pleiades Publishing},
month = {apr},
url = {https://doi.org/10.3103/S0096392517020043},
number = {2},
pages = {85--90},
doi = {10.3103/S0096392517020043}
}
Cite this
MLA
Copy
Pavlova, E. R., et al. “Nuclei deformation in HaCaT keratinocytes cultivated on aligned fibrous substrates.” Moscow University Biological Sciences Bulletin, vol. 72, no. 2, Apr. 2017, pp. 85-90. https://doi.org/10.3103/S0096392517020043.
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