Phytochemical and chromatographic analysis of flavanoid fraction isolated from methanoloc extract of Pterocarpus marsupium

Medicinal plants are considered as rich sources of active components which can be used in drug development. Pterocarpus marsupium is a medicinal plant known for therapeutic efficacy against Diabetes mellitus and inflammatory conditions. The proximate analysis, phytochemical screening, GCMS analysis and the HPTLC analysis of flavonoid fraction isolated from methanolic extract of Pterocarpus marsupium were performed in the present study. The total ash, crude protein, crude fat, crude fibre, acid insoluble ash, carbohydrate content, calcium and phosphorus content were all measured. Standard techniques were used to examine the methanolic leaf extract for various phytochemical components. The presence of alkaloid, saponins, phytosterols, phenolic compounds, flavanoids, terpenoids and cardiac glycosides was revealed from the methanolic extract of P. marsupium. TLC analysis of crude methanolic extract of P. marsupium was conducted to study the separation pattern with different solvent systems and solvent combination with effective separation of components. The HPTLC finger print profiling of flavonoid fraction of methanolic extract of leaf of P. marsupium were also conducted. The GC-MS analysis revealed the composition of 13-Docosenamide and 1-Bromo-4- bromomethyldecane.