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Open access
LLC Numicom
Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)
,  volume 23 ,  issue 6 ,  pages 129-136

Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens

Publication type: Journal Article
Publication date: 2025-01-15
LLC Numicom
scimago Q4
SJR: 0.162
CiteScore: 1.3
Impact factor: —
ISSN: 20733046, 26190494
Abstract

Relevance. Obtaining clean lysis spots, with tolerable presence of individual colonies of secondary growth, in spot tests is a criterion for assessing the effectiveness of therapeutic bacteriophages. At the same time, a number of mechanisms are known by which phage lysis of bacteria on lawns occurs without being accompanied by virus replication.Aim. To assess the ratio of spot test results and tests based on the detection of negative bacteriophage colonies.Conclusion. Data on 43 bacteriophages extracted from 21 articles were analyzed. Within the studied sample, the observation of clean lysis zones in 94% of cases corresponded to successful virus replication. For a number of Escherichia coli bacteriophages, a greater number of spot tests of the "++++" category were detected compared to the number of strains supporting virus replication, which, within the framework of assessing the lytic activity of a therapeutic bacteriophage, can be characterized as false positive spot test results. In general, the observation of clear lysis spots on the spot test in most cases indicates bacteriophage replication, which allows us to consider the spot test method as indicative, but requiring subsequent validation by more complex methods characterizing the efficiency of phage replication.

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Pchelin I. M. et al. Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens // Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika). 2025. Vol. 23. No. 6. pp. 129-136.
GOST all authors (up to 50) Copy
Pchelin I. M., Azarov D., Dedik V. A., Kushnirenko D. A., Aslanov B. I., Goncharov A. E., Lioznov D. Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens // Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika). 2025. Vol. 23. No. 6. pp. 129-136.
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RIS Copy
TY - JOUR
DO - 10.31631/2073-3046-2024-23-6-129-136
UR - https://www.epidemvac.ru/jour/article/view/2128
TI - Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens
T2 - Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)
AU - Pchelin, Ivan M
AU - Azarov, Daniil
AU - Dedik, V. A.
AU - Kushnirenko, Daria A.
AU - Aslanov, B I
AU - Goncharov, Artemiy E.
AU - Lioznov, Dmitry
PY - 2025
DA - 2025/01/15
PB - LLC Numicom
SP - 129-136
IS - 6
VL - 23
SN - 2073-3046
SN - 2619-0494
ER -
BibTex |
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BibTex (up to 50 authors) Copy
@article{2025_Pchelin,
author = {Ivan M Pchelin and Daniil Azarov and V. A. Dedik and Daria A. Kushnirenko and B I Aslanov and Artemiy E. Goncharov and Dmitry Lioznov},
title = {Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens},
journal = {Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika)},
year = {2025},
volume = {23},
publisher = {LLC Numicom},
month = {jan},
url = {https://www.epidemvac.ru/jour/article/view/2128},
number = {6},
pages = {129--136},
doi = {10.31631/2073-3046-2024-23-6-129-136}
}
MLA
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Pchelin, Ivan M., et al. “Evaluation of the Ratio of Spot Tests and Plaque-Forming Activity Tests of Bacteriophages of Prevalent Pathogens.” Epidemiology and Vaccinal Prevention (Epidemiologiya i Vaktsinoprofilaktika), vol. 23, no. 6, Jan. 2025, pp. 129-136. https://www.epidemvac.ru/jour/article/view/2128.