Open Access
Frontiers in Immunology, volume 12
Detection and Neutralization of SARS-CoV-2 Using Non-conventional Variable Lymphocyte Receptor Antibodies of the Evolutionarily Distant Sea Lamprey
Leslie Y. T. Leung
1
,
Srijit Khan
1
,
Patrick Budylowski
2
,
Zhijie Li
3, 4
,
Sofiya Goroshko
1
,
Yanling Liu
1
,
Shilan Dong
1
,
James R. Carlyle
1
,
James M. Rini
3, 4
,
Mario Ostrowski
1, 2
,
Götz R. A. Ehrhardt
1
1
Department of Immunology, University of Toronto, Canada
|
2
Department of Medicine, University of Toronto, Canada
|
3
Department of Biochemistry, University of Toronto, Canada
|
4
Department of Molecular Genetics, University of Toronto, Canada
|
Publication type: Journal Article
Publication date: 2021-06-21
Journal:
Frontiers in Immunology
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 7.3
ISSN: 16643224
Immunology
Immunology and Allergy
Abstract
SARS-CoV-2 is a newly emerged betacoronavirus and the causative agent for the COVID-19 pandemic. Antibodies recognizing the viral spike protein are instrumental in natural and vaccine-induced immune responses to the pathogen and in clinical diagnostic and therapeutic applications. Unlike conventional immunoglobulins, the variable lymphocyte receptor antibodies of jawless vertebrates are structurally distinct, indicating that they may recognize different epitopes. Here we report the isolation of monoclonal variable lymphocyte receptor antibodies from immunized sea lamprey larvae that recognize the spike protein of SARS-CoV-2 but not of other coronaviruses. We further demonstrate that these monoclonal variable lymphocyte receptor antibodies can efficiently neutralize the virus and form the basis of a rapid, single step SARS-CoV-2 detection system. This study provides evidence for monoclonal variable lymphocyte receptor antibodies as unique biomedical research and potential clinical diagnostic reagents targeting SARS-CoV-2.
Top-30
Citations by journals
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1
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Journal of Immunology
1 publication, 20%
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Fish and Shellfish Immunology
1 publication, 20%
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Russian Chemical Reviews
1 publication, 20%
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Developmental and Comparative Immunology
1 publication, 20%
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1
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Citations by publishers
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1
2
3
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Elsevier
3 publications, 60%
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The American Association of Immunologists
1 publication, 20%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 20%
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1
2
3
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Leung L. Y. T. et al. Detection and Neutralization of SARS-CoV-2 Using Non-conventional Variable Lymphocyte Receptor Antibodies of the Evolutionarily Distant Sea Lamprey // Frontiers in Immunology. 2021. Vol. 12.
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Leung L. Y. T., Khan S., Budylowski P., Li Z., Goroshko S., Liu Y., Dong S., Carlyle J. R., Rini J. M., Ostrowski M., Ehrhardt G. R. A. Detection and Neutralization of SARS-CoV-2 Using Non-conventional Variable Lymphocyte Receptor Antibodies of the Evolutionarily Distant Sea Lamprey // Frontiers in Immunology. 2021. Vol. 12.
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TY - JOUR
DO - 10.3389/fimmu.2021.659071
UR - https://doi.org/10.3389/fimmu.2021.659071
TI - Detection and Neutralization of SARS-CoV-2 Using Non-conventional Variable Lymphocyte Receptor Antibodies of the Evolutionarily Distant Sea Lamprey
T2 - Frontiers in Immunology
AU - Leung, Leslie Y. T.
AU - Khan, Srijit
AU - Budylowski, Patrick
AU - Li, Zhijie
AU - Goroshko, Sofiya
AU - Liu, Yanling
AU - Dong, Shilan
AU - Carlyle, James R.
AU - Rini, James M.
AU - Ostrowski, Mario
AU - Ehrhardt, Götz R. A.
PY - 2021
DA - 2021/06/21 00:00:00
PB - Frontiers Media S.A.
VL - 12
SN - 1664-3224
ER -
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@article{2021_Leung,
author = {Leslie Y. T. Leung and Srijit Khan and Patrick Budylowski and Zhijie Li and Sofiya Goroshko and Yanling Liu and Shilan Dong and James R. Carlyle and James M. Rini and Mario Ostrowski and Götz R. A. Ehrhardt},
title = {Detection and Neutralization of SARS-CoV-2 Using Non-conventional Variable Lymphocyte Receptor Antibodies of the Evolutionarily Distant Sea Lamprey},
journal = {Frontiers in Immunology},
year = {2021},
volume = {12},
publisher = {Frontiers Media S.A.},
month = {jun},
url = {https://doi.org/10.3389/fimmu.2021.659071},
doi = {10.3389/fimmu.2021.659071}
}