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Horticulturae, volume 8, issue 10, pages 864

Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family

Kozar Elena Victorovna ¹

Kozar Elena G ¹

Domblides Elena ¹

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Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI FSVC), Selektsionnaya St., 14, VNIISSOK, Odintsovo Reg., 143072 Moscow, Russia |

Publication type: Journal Article

Publication date: 2022-09-22

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Horticulturae

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Impact factor: 3.1

ISSN: 23117524

DOI: 10.3390/horticulturae8100864

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Plant Science

Horticulture

Abstract

Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation method. We found that the new method allows an increase in the percentage of microspores at the embryogenic stage of development in the culture. In the spring rapeseed ‘Ratnik’ culture the percentage of microspores increases from 66.7% to 73%, and in the European radish ‘RBK’ from 34% to 61.9%. Moreover, the new method of microspore isolation made it possible to expand the range of linear bud sizes (from 3.5–4.0 to 3.0–4.5 mm for spring rapeseed ‘Ratnik’) suitable for IMC technology. In addition, the new method of microspore isolation reduced the debris in the preparation of spring rapeseed ‘Ratnik’ and European radish ‘RBK’ by 2.4 and 15 times, respectively. The best results were shown on Sareptian mustard No. 72, where the yield of embryoids increased by 7.5 times. Remarkably, the new method of microspore isolation allowed us to obtain the first embryoids of red cabbage No. 428, whereas no embryoids were obtained using the standard method of microspore isolation. In summary, the new method of microspore isolation allows an increase in the efficiency of IMC technology for Brassicaceae family crops.

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	1
Vegetable crops of Russia	Vegetable crops of Russia, 1, 50% Vegetable crops of Russia 1 publication, 50%
Plant Cell, Tissue and Organ Culture	Plant Cell, Tissue and Organ Culture, 1, 50% Plant Cell, Tissue and Organ Culture 1 publication, 50%
	1

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Federal Scientific Center for Vegetable Growing	Federal Scientific Center for Vegetable Growing, 1, 50% Federal Scientific Center for Vegetable Growing 1 publication, 50%
Springer Nature	Springer Nature, 1, 50% Springer Nature 1 publication, 50%
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Kozar E. V., Kozar E. G., Domblides E. Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family // Horticulturae. 2022. Vol. 8. No. 10. p. 864.

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TY - JOUR

DO - 10.3390/horticulturae8100864

UR - https://doi.org/10.3390%2Fhorticulturae8100864

TI - Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family

T2 - Horticulturae

AU - Kozar, Elena G

AU - Kozar, Elena Victorovna

AU - Domblides, Elena

PY - 2022

DA - 2022/09/22 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 864

IS - 10

VL - 8

SN - 2311-7524

ER -

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@article{2022_Kozar,

author = {Elena G Kozar and Elena Victorovna Kozar and Elena Domblides},

title = {Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family},

journal = {Horticulturae},

year = {2022},

volume = {8},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {sep},

url = {https://doi.org/10.3390%2Fhorticulturae8100864},

number = {10},

pages = {864},

doi = {10.3390/horticulturae8100864}

}

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Kozar, Elena Victorovna, et al. “Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family.” Horticulturae, vol. 8, no. 10, Sep. 2022, p. 864. https://doi.org/10.3390%2Fhorticulturae8100864.

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Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Horticulturae

Quartile SCImago

Quartile WOS

Impact factor

3.1

ISSN

23117524 (Electronic)

Labs

Laboratory of reproductive biotechnology in the breeding of agricultural plants

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