Open Access

Journal of Marine Science and Engineering, volume 10, issue 5, pages 707

Oxidative Stress in Far Eastern Mussel Mytilus trossulus (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs)

Dovzhenko Nadezda Vladimirovna ¹

Chelomin Victor Pavlovich ¹

Mazur Andrey Alexandrovich ¹

Kukla Sergey Petrovich ¹

Slobodskova Valentina Vladimirovna ¹

Istomina Aleksandra Anatolievna ¹

Zhukovskaya Avianna Fayazovna ¹

Hide authors affiliations Show authors affiliations: 1 affiliation

Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences, 690041 Vladivostok, Russia |

Publication type: Journal Article

Publication date: 2022-05-22

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Journal of Marine Science and Engineering

Quartile SCImago

Quartile WOS

Impact factor: 2.9

ISSN: 20771312

DOI: 10.3390/jmse10050707

Copy DOI

Civil and Structural Engineering

Water Science and Technology

Ocean Engineering

Abstract

The ingress of nanoparticles of metal oxides and microfragments of synthetic polymers (microplastics) into a marine environment causes unpredictable consequences. The effects of such particles cannot be predicted due to a lack of ecotoxicological information. In this research, a series of laboratory experiments were conducted on the combined effects of CuO-nanoparticles (CuO-NPs) and polystyrene microspheres (µPSs) on the development of oxidative stress processes in the marine filter-feeder mollusk Mytilus trossulus. Biomarkers of oxidative stress, including the lysosome membrane stability of hematocytes (LMS), the index of antioxidant activity (IAA), the levels of malonaldehyde (MDA) and protein carbonyls (PCs), and DNA damage in digestive gland cells, were measured after 5 days of exposure. Based on a battery of biochemical markers, it was shown that oxidative stress was induced at varying degrees in the experimental mollusks when exposed to CuO-NPs and µPSs both separately and in combination. In contrast, the single-treatment effect on the lysosomal membrane was enhanced by the combined CuO-NPs and µPSs (from 77.14 ± 8.56 to 42 ± 4.26 min). In addition, exposure to both the compounds alone and in combination decreased the IAA (from 22.87 ± 1.25, to 19.55 ± 0.21, 10.73 ± 0.53, and 12.06 ± 1.62 nM/mg protein, respectively). The PC level significantly increased only after CuO-NP exposure (from 0.496 ± 0.02 to 0.838 ± 0.03 μM/mg protein). Furthermore, the results showed that the investigated particles, both alone and in combination, promoted DNA damage in digestive gland cells (from 2.02 ± 0.52 to 5.15 ± 0.37, 18.29 ± 2.14, and 10.72 ± 2.53%, respectively), indicating that these compounds are genotoxic. Overall, the results obtained suggest that oxidative stress is the leading factor in the negative effects of CuO-NPs and µPSs. Considering the exceptional role of genome integrity in the functioning of biological systems, the revealed damages in the DNA molecule structure should be attributed to the most important manifestations of the toxicity of these two forms of marine pollution.

By date By citations

Citations by journals

	1
Environmental Pollution	Environmental Pollution, 1, 20% Environmental Pollution 1 publication, 20%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 20% International Journal of Molecular Sciences 1 publication, 20%
Toxics	Toxics, 1, 20% Toxics 1 publication, 20%
Journal of Trace Elements in Medicine and Biology	Journal of Trace Elements in Medicine and Biology, 1, 20% Journal of Trace Elements in Medicine and Biology 1 publication, 20%
	1

Citations by publishers

	1 2
Elsevier	Elsevier, 2, 40% Elsevier 2 publications, 40%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 40% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 40%
Research Square Platform LLC	Research Square Platform LLC, 1, 20% Research Square Platform LLC 1 publication, 20%
	1 2

We do not take into account publications that without a DOI.
Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
Statistics recalculated weekly.

{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2023,2024],"ids":[0,0],"codes":[0,0],"imageUrls":["",""],"datasets":[{"label":"Citations number","data":[3,2],"backgroundColor":["#3B82F6","#3B82F6"],"percentage":["60","40"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Environmental Pollution","International Journal of Molecular Sciences","Toxics","Journal of Trace Elements in Medicine and Biology"],"ids":[13439,14627,22117,17461],"codes":[0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[20,20,20,20],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","Research Square Platform LLC"],"ids":[17,202,9418],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp",""],"datasets":[{"label":"","data":[2,2,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[40,40,20],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}

Publication PDF

Metrics

Cite this

GOST |

Cite this

GOST Copy

Dovzhenko N. V. et al. Oxidative Stress in Far Eastern Mussel Mytilus trossulus (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) // Journal of Marine Science and Engineering. 2022. Vol. 10. No. 5. p. 707.

GOST all authors (up to 50) Copy

Dovzhenko N. V., Chelomin V. P., Mazur A. A., Kukla S. P., Slobodskova V. V., Istomina A. A., Zhukovskaya A. F. Oxidative Stress in Far Eastern Mussel Mytilus trossulus (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs) // Journal of Marine Science and Engineering. 2022. Vol. 10. No. 5. p. 707.

RIS |

Cite this

RIS Copy

TY - JOUR

DO - 10.3390/jmse10050707

UR - https://doi.org/10.3390%2Fjmse10050707

TI - Oxidative Stress in Far Eastern Mussel Mytilus trossulus (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs)

T2 - Journal of Marine Science and Engineering

AU - Chelomin, Victor Pavlovich

AU - Kukla, Sergey Petrovich

AU - Slobodskova, Valentina Vladimirovna

AU - Istomina, Aleksandra Anatolievna

AU - Zhukovskaya, Avianna Fayazovna

AU - Dovzhenko, Nadezda Vladimirovna

AU - Mazur, Andrey Alexandrovich

PY - 2022

DA - 2022/05/22 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 707

IS - 5

VL - 10

SN - 2077-1312

ER -

BibTex |

Cite this

BibTex Copy

@article{2022_Dovzhenko,

author = {Victor Pavlovich Chelomin and Sergey Petrovich Kukla and Valentina Vladimirovna Slobodskova and Aleksandra Anatolievna Istomina and Avianna Fayazovna Zhukovskaya and Nadezda Vladimirovna Dovzhenko and Andrey Alexandrovich Mazur},

title = {Oxidative Stress in Far Eastern Mussel Mytilus trossulus (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs)},

journal = {Journal of Marine Science and Engineering},

year = {2022},

volume = {10},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {may},

url = {https://doi.org/10.3390%2Fjmse10050707},

number = {5},

pages = {707},

doi = {10.3390/jmse10050707}

}

MLA

Cite this

MLA Copy

Dovzhenko, Nadezda Vladimirovna, et al. “Oxidative Stress in Far Eastern Mussel Mytilus trossulus (Gould, 1850) Exposed to Combined Polystyrene Microspheres (µPSs) and CuO-Nanoparticles (CuO-NPs).” Journal of Marine Science and Engineering, vol. 10, no. 5, May. 2022, p. 707. https://doi.org/10.3390%2Fjmse10050707.

Found error?

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Journal of Marine Science and Engineering

Quartile SCImago

Quartile WOS

Impact factor

2.9

ISSN

20771312 (Electronic)

Labs

Laboratory of Marine Ecotoxicology

Profiles