Open Access
Life, volume 11, issue 1, pages 20
Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.)
Publication type: Journal Article
Publication date: 2020-12-31
General Biochemistry, Genetics and Molecular Biology
Ecology, Evolution, Behavior and Systematics
Paleontology
Space and Planetary Science
Abstract
The process of embryogenesis in isolated microspore culture was studied in eight carrot accessions of different origin. The ½NLN-13 medium supplemented with 0.2 mg/L 2,4D and 0.2mg/L kinetin was used to induce embryogenesis. The temperature treatment was performed at 5–6 °C for three days, followed by cultivation at 25 °C in darkness. As was shown, the first embryogenesis was only observed in microspores at the late vacuolated stage when the nucleus moved from the center to one pole following the long cell axis. Depending on the nucleus position, the microspore can divide into two equal or two different sized cells. Following divisions occurred either in one of these cells or in two. However, microspores that divided into two unequal cells were morphologically different form bi-cellular pollen grain. Embryogenic divisions in bi-cellular pollen grains were not observed. First divisions began by the third day of cultivation, and continued until the globular embryoid stage that was well-seen after the fourth week of cultivation. The already-formed embryoids can develop the secondary embryoids on their surface. Depending on the genotype, up to 1000 secondary embryoids can be produced from one embryoid in the liquid MSm medium supplemented with 0.1 mg/L of kinetin for regeneration. All carrot accessions studied were split into three groups: responsive genotypes, weakly responsive genotypes, and reluctant genotypes. The highest yield was 53 initial embryoids per a 6 cm diameter petri dish. Thus, the Nantskaya 4 cultivar totally produced 256 initial embryoids, out of which 94 developed into green plantlets and 162 into albino plantlets, whereas 97 initial embryoids with 45 albino plantlets formed from them were obtained from Chantenay cultivar.
Citations by journals
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2
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Protoplasma
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Protoplasma
2 publications, 18.18%
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Vegetable crops of Russia
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2 publications, 18.18%
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Plants
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1 publication, 9.09%
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Foods and Raw Materials
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Agronomy Journal
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1 publication, 9.09%
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Agronomy
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1 publication, 9.09%
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Plant Cell, Tissue and Organ Culture
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1 publication, 9.09%
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1
2
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Citations by publishers
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1
2
3
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Springer Nature
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Springer Nature
3 publications, 27.27%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 18.18%
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Federal Scientific Center for Vegetable Growing
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Federal Scientific Center for Vegetable Growing
2 publications, 18.18%
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Elsevier
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Elsevier
1 publication, 9.09%
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Kemerovo State University
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Kemerovo State University
1 publication, 9.09%
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American Society of Agronomy
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American Society of Agronomy
1 publication, 9.09%
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1
2
3
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Shmykova N. et al. Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.) // Life. 2020. Vol. 11. No. 1. p. 20.
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Shmykova N., Domblides E., Vjurtts T., Domblides A. Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.) // Life. 2020. Vol. 11. No. 1. p. 20.
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TY - JOUR
DO - 10.3390/life11010020
UR - https://doi.org/10.3390%2Flife11010020
TI - Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.)
T2 - Life
AU - Shmykova, Natalia
AU - Vjurtts, Tatiana
AU - Domblides, Arthur
AU - Domblides, Elena
PY - 2020
DA - 2020/12/31 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 20
IS - 1
VL - 11
SN - 2075-1729
ER -
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@article{2020_Shmykova,
author = {Natalia Shmykova and Tatiana Vjurtts and Arthur Domblides and Elena Domblides},
title = {Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.)},
journal = {Life},
year = {2020},
volume = {11},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {dec},
url = {https://doi.org/10.3390%2Flife11010020},
number = {1},
pages = {20},
doi = {10.3390/life11010020}
}
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Shmykova, Natalia, et al. “Haploid Embryogenesis in Isolated Microspore Culture of Carrots (Daucus carota L.).” Life, vol. 11, no. 1, Dec. 2020, p. 20. https://doi.org/10.3390%2Flife11010020.