A New Method for Accelerated Aging of Nanoparticles to Assess the Colloidal Stability of Albumin-Coated Magnetic Nanoparticles

Boris Nikolaev ¹

Ludmila Yakovleva ¹

Viacheslav Fedorov ^{1, 2, 3}

Natalia M Yudintceva ^{1, 2}

Daria Tarasova ¹

Elizaveta Perepelitsa ⁴

Anastasia Dmitrieva ¹

Maksim I Sulatsky ¹

Sivaprakash Srinivasan ⁵

Shirish H. Sonawane ⁵

Anusha Srivastava ⁶

Sharad Gupta ⁶

A. Sonawane ⁶

Stephanie E. Combs ⁷

M. B. Shevtsov ^{1, 2, 7}

Show full list: 15 authors

Hide authors affiliations

Laboratory of Biomedical Nanotechnologies, Institute of Cytology of the Russian Academy of Sciences (RAS), 194064 Saint Petersburg, Russia |

Personalized Medicine Centre, Almazov National Medical Research Centre, 197341 Saint Petersburg, Russia |

Department of Inorganic Chemistry and Biophysics, Saint-Petersburg State University of Veterinary Medicine, 196084 Saint-Petersburg, Russia

⁴

Research Center of Neurology, 125367 Moscow, Russia |

⁵

Department of Chemical Engineering, National Institute of Technology, Warangal 506004, Telangana State, India |

⁶

Department of Biosciences & Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552, Madhya Pradesh, India

⁷

Department of Radiation Oncology, Technische Universität München (TUM), Klinikum rechts der Isar, 81675 Munich, Germany

Publication type: Journal Article

Publication date: 2025-03-21

MDPI

Journal: Nanomaterials

scimago Q1

wos Q2

SJR: 0.798

CiteScore: 8.5

Impact factor: 4.4

ISSN: 20794991

DOI: 10.3390/nano15070475

Copy DOI

Abstract

The colloidal long-storage stability of nanosized drugs is a crucial factor for pharmacology, as they require much time for robust estimation. The application of bioavailable magnetic nanosuspensions in theranostics is limited by incomplete information about their colloidal stability in the internal media of human organisms. A method for the accelerated temperature stress “aging” of magnetic nanosized suspensions is proposed for the rapid assessment and prediction of the colloidal stability over time of nanosized iron oxide suspensions stabilized by albumin HSA. Colloidal stability is assessed using dynamic light scattering (DLS), fluorescence spectroscopy, electrophoresis, and ion monitoring methods during short- and long-term storage. Rapid assessment is achieved by short high-temperature (70 °C) processing of carboxymethyl-dextran-coated nanosol in the presence of albumin. The role of albumin in the sustained stability of superparamagnetic iron oxide particles (SPIONs) was studied under conditions mimicking blood plasma (pH = 7.4) and endolysosomal cell compartments (pH = 5.5). According to the fluorescence quenching and DLS data, colloidal stability is ensured by the formation of an HSA corona on carboxymethyl-dextran-coated SPIONs and their process of clustering. In the presence of albumin, the colloidal stability of nanoparticles is shown to increase from 80 to 121 days at a storage temperature of 8 °C The prognostic shelf life of magnetic nanosol is estimated by calculating the Van’t Hoff’s relation for the rate of chemical reactions. The validity of using the Van’t Hoff’s rule is confirmed by the agreement of the calculated activation energy at 8 °C and 70 °C. The developed method of the accelerated aging of nanoparticles can not only be employed for the estimation of the shelf life of magnetic nanoparticles coated with HSA in vitro but also for assessing the stability of SPIONs applied in vivo.