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Pathogens
,  volume 11 ,  issue 2 ,  pages 245

Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil

Thiara Manuele Alves de Souza 1
, 
Raquel Curtinhas De Lima 1
, 
Victor Edgar Fiestas Solórzano 1
, 
Paulo Vieira Damasco 2, 3, 4
, 
Luiz José De Souza 5
, 
Juan Camilo Sánchez Arcila 1
, 
Gabriel Macedo Costa Guimarães 1
, 
Iury Amancio Paiva 1
, 
Monique Da Rocha Queiroz Lima 1
, 
Fernanda de Bruycker Nogueira 6
, 
Larissa Cristina Teixeira Tomé 1
, 
Mariana Rosa Inácio Coelho 1
, 
Sandro Patroca Da Silva 7
, 
Luzia Maria de Oliveira-Pinto 1
, 
Elzinandes Leal De Azeredo 1
, 
Flavia Barreto dos Santos 1
1
 
Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro 21040-360, Brazil | 
2
 
Rio-Laranjeiras Hospital, Rio de Janeiro 22240-000, Brazil | 
3
 
4
 
Pedro Ernesto University Hospital, University of the State of Rio de Janeiro, Rio de Janeiro 20551-030, Brazil | 
5
 
Plantadores de Cana Hospital, Campos dos Goytacazes, Rio de Janeiro 28025-496, Brazil | 
6
 
Flavivirus Laboratory, Oswaldo Cruz Institute, Rio de Janeiro 21040-360, Brazil | 
7
 
Publication type: Journal Article
Publication date: 2022-02-14
MDPI
scimago Q1
wos Q2
SJR: 0.949
CiteScore: 6.8
Impact factor: 3.3
ISSN: 20760817
Molecular Biology
Microbiology (medical)
Infectious Diseases
General Immunology and Microbiology
Immunology and Allergy
Abstract

The co-circulation of chikungunya virus (CHIKV), dengue virus (DENV) and Zika virus (ZIKV) in Rio de Janeiro (RJ), Brazil, caused a challenging triple epidemic, as they share similar clinical signs and symptoms and geographical distribution. Here, we aimed to investigate the clinical and laboratorial aspects of chikungunya suspected cases assisted in RJ during the 2018 outbreak, focusing on the differential diagnosis with dengue and zika. All suspected cases were submitted to molecular and/or serological differential diagnostic approaches to arboviruses. A total of 242 cases suspected of arbovirus infection were investigated and 73.6% (178/242) were molecular and/or serologically confirmed as chikungunya. In RT-qPCR confirmed cases, cycle threshold (Ct) values ranged from 15.46 to 35.13, with acute cases presenting lower values. Chikungunya cases were mainly in females (64%) and the most frequently affected age group was adults between 46 to 59 years old (27%). Polyarthralgia affected 89% of patients, especially in hands and feet. No dengue virus (DENV) and Zika virus (ZIKV) infections were confirmed by molecular diagnosis, but 9.5% (23/242) had serological evidence of DENV exposure by the detection of specific anti-DENV IgM or NS1, and 42.7% (76/178) of chikungunya positive cases also presented recent DENV exposure reflected by a positive anti-DENV IgM or NS1 result. A significantly higher frequency of arthritis (p = 0.023) and limb edema (p < 0.001) was found on patients with CHIKV monoinfection compared to dengue patients and patients exposed to both viruses. Lastly, phylogenetic analysis showed that the chikungunya cases were caused by the ECSA genotype. Despite the triple arboviruses’ epidemic in the state of RJ, most patients with fever and arthralgia investigated here were diagnosed as chikungunya cases, and the incidence of CHIKV/DENV co-detection was higher than that reported in other studies.

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GOST Copy
de Souza T. M. A. et al. Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil // Pathogens. 2022. Vol. 11. No. 2. p. 245.
GOST all authors (up to 50) Copy
de Souza T. M. A., De Lima R. C., Solórzano V. E. F., Damasco P. V., De Souza L. J., Sánchez Arcila J. C., Guimarães G. M. C., Paiva I. A., Da Rocha Queiroz Lima M., de Bruycker Nogueira F., Tomé L. C. T., Coelho M. R. I., Silva S. P. D., de Oliveira-Pinto L. M., De Azeredo E. L., Santos F. B. D. Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil // Pathogens. 2022. Vol. 11. No. 2. p. 245.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.3390/pathogens11020245
UR - https://doi.org/10.3390/pathogens11020245
TI - Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil
T2 - Pathogens
AU - de Souza, Thiara Manuele Alves
AU - De Lima, Raquel Curtinhas
AU - Solórzano, Victor Edgar Fiestas
AU - Damasco, Paulo Vieira
AU - De Souza, Luiz José
AU - Sánchez Arcila, Juan Camilo
AU - Guimarães, Gabriel Macedo Costa
AU - Paiva, Iury Amancio
AU - Da Rocha Queiroz Lima, Monique
AU - de Bruycker Nogueira, Fernanda
AU - Tomé, Larissa Cristina Teixeira
AU - Coelho, Mariana Rosa Inácio
AU - Silva, Sandro Patroca Da
AU - de Oliveira-Pinto, Luzia Maria
AU - De Azeredo, Elzinandes Leal
AU - Santos, Flavia Barreto dos
PY - 2022
DA - 2022/02/14
PB - MDPI
SP - 245
IS - 2
VL - 11
PMID - 35215188
SN - 2076-0817
ER -
BibTex |
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BibTex (up to 50 authors) Copy
@article{2022_de Souza,
author = {Thiara Manuele Alves de Souza and Raquel Curtinhas De Lima and Victor Edgar Fiestas Solórzano and Paulo Vieira Damasco and Luiz José De Souza and Juan Camilo Sánchez Arcila and Gabriel Macedo Costa Guimarães and Iury Amancio Paiva and Monique Da Rocha Queiroz Lima and Fernanda de Bruycker Nogueira and Larissa Cristina Teixeira Tomé and Mariana Rosa Inácio Coelho and Sandro Patroca Da Silva and Luzia Maria de Oliveira-Pinto and Elzinandes Leal De Azeredo and Flavia Barreto dos Santos},
title = {Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil},
journal = {Pathogens},
year = {2022},
volume = {11},
publisher = {MDPI},
month = {feb},
url = {https://doi.org/10.3390/pathogens11020245},
number = {2},
pages = {245},
doi = {10.3390/pathogens11020245}
}
MLA
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MLA Copy
de Souza, Thiara Manuele Alves, et al. “Was It Chikungunya? Laboratorial and Clinical Investigations of Cases Occurred during a Triple Arboviruses’ Outbreak in Rio de Janeiro, Brazil.” Pathogens, vol. 11, no. 2, Feb. 2022, p. 245. https://doi.org/10.3390/pathogens11020245.