Open Access

Pharmaceutics

, volume 15 , issue 1 , pages 8

Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells

Vera Vysochinskaya ^{1, 2}

Sergey Mikhailovich Shishlyannikov ^{1, 2}

Yana A. Zabrodskaya ^{1, 2}

Elena Shmendel ³

Sergey Klotchenko ¹

Olga Dobrovolskaya ¹

Nina Gavrilova ¹

Darya Makarova ³

Marina Plotnikova ¹

Ekaterina Elpaeva ¹

Andrey Gorshkov ¹

Dmitry Moshkoff ^{2, 4}

Michael Maslov ³

Andrey Vasin ^{1, 2}

Hide authors affiliations Show authors affiliations: 4 affiliations

Smorodintsev Research Institute of Influenza, 15/17 Ulitsa Professora Popova, 197376 St. Petersburg, Russia |

Institute of Biomedical Systems and Biotechnology, Peter the Great Saint Petersburg Polytechnic University, 29 Ulitsa Polytechnicheskaya, 194064 St. Petersburg, Russia |

Lomonosov Institute of Fine Chemical Technologies, MIREA—Russian Technological University, 86 Vernadsky Ave, 119571 Moscow, Russia |

⁴

Global Virus Network (GVN), 725 W Lombard St, Baltimore, MD 21201, USA |

Publication type: Journal Article

Publication date: 2022-12-20

MDPI

Pharmaceutics

scimago Q1

wos Q1

SJR: 1.075

CiteScore: 10.0

Impact factor: 5.5

ISSN: 19994923

DOI: 10.3390/pharmaceutics15010008

Copy DOI

PubMed ID: 36678637

Pharmaceutical Science

Abstract

The design of cationic liposomes for efficient mRNA delivery can significantly improve mRNA-based therapies. Lipoplexes based on polycationic lipid 1,26-bis(cholest-5-en-3β-yloxycarbonylamino)-7,11,16,20-tetraazahexacosane tetrahydrochloride (2X3) and helper lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) were formulated in different molar ratios (1:1, 1:2, 1:3) to efficiently deliver model mRNAs to BHK-21 and A549. The objective of this study was to examine the effect of 2X3-DOPE composition as well as lipid-to-mRNA ratio (amino-to-phosphate group ratio, N/P) on mRNA transfection. We found that lipoplex-mediated transfection efficiency depends on both liposome composition and the N/P ratio. Lipoplexes with an N/P ratio of 10/1 showed nanometric hydrodynamic size, positive ζ potential, maximum loading, and transfection efficiency. Liposomes 2X3-DOPE (1:3) provided the superior delivery of both mRNA coding firefly luciferase and mRNA-eGFP into BHK-21 cells and A549 cells, compared with commercial Lipofectamine MessengerMax.

Found

7 citations

Maslov Mikhail

DSc in Chemistry, associate professor

88 publications, 860 citations, 13 reviews

h-index: 15

MIREA — Russian Technological University

5 citations

Shmendel Elena

PhD

39 publications, 420 citations

h-index: 12

MIREA — Russian Technological University

2 citations

Zabrodskaya Yana

PhD in Physics and Mathematics

68 publications, 386 citations

h-index: 11

Peter the Great St. Petersburg Polytechnic University

Smorodintsev Research Institute of Influenza

2 citations

Vasin Andrey

DSc in Biological/biomedical sciences, associate professor, professor of the Russian Academy of Sciences

98 publications, 906 citations

h-index: 17

Peter the Great St. Petersburg Polytechnic University

Smorodintsev Research Institute of Influenza

Research interests

Molecular Virology

Molecular biology

RNA drugs and vaccines

1 citation

Dmitriev Sergey

PhD

105 publications, 3 853 citations, 8 reviews

h-index: 38

Research interests

1 citation

Markov Oleg

22 publications, 460 citations, 1 review

h-index: 11

Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences

1 citation

Golyshev Victor

🥼 🤝

PhD in Physics and Mathematics, senior lecturer

32 publications, 143 citations

h-index: 8

Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences

Novosibirsk State University

Research interests

Atomic force microscopy

Biophysics

Biopolymers - structure

Circular and magnetic circular dichroism spectroscopy

Structural Bioinformatics

Structural Biology

Thermal analysis

Thermodynamics

1 citation

Stepanov Grigory

🤝

PhD in Chemistry

51 publications, 580 citations, 2 reviews

h-index: 11

Institute of Chemical Biology and Fundamental Medicine of the Siberian Branch of the Russian Academy of Sciences

RNA drugs and vaccines

1 citation

Saraf Shubhini

156 publications, 3 032 citations, 24 reviews

h-index: 29

Babasaheb Bhimrao Ambedkar University

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Vysochinskaya V. et al. Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells // Pharmaceutics. 2022. Vol. 15. No. 1. p. 8.

GOST all authors (up to 50) Copy

Vysochinskaya V., Shishlyannikov S. M., Zabrodskaya Y. A., Shmendel E., Klotchenko S., Dobrovolskaya O., Gavrilova N., Makarova D., Plotnikova M., Elpaeva E., Gorshkov A., Moshkoff D., Maslov M., Vasin A. Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells // Pharmaceutics. 2022. Vol. 15. No. 1. p. 8.

RIS |

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RIS Copy

TY - JOUR

DO - 10.3390/pharmaceutics15010008

UR - https://www.mdpi.com/1999-4923/15/1/8

TI - Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells

T2 - Pharmaceutics

AU - Vysochinskaya, Vera

AU - Shishlyannikov, Sergey Mikhailovich

AU - Zabrodskaya, Yana A.

AU - Shmendel, Elena

AU - Klotchenko, Sergey

AU - Dobrovolskaya, Olga

AU - Gavrilova, Nina

AU - Makarova, Darya

AU - Plotnikova, Marina

AU - Elpaeva, Ekaterina

AU - Gorshkov, Andrey

AU - Moshkoff, Dmitry

AU - Maslov, Michael

AU - Vasin, Andrey

PY - 2022

DA - 2022/12/20

PB - MDPI

SP - 8

IS - 1

VL - 15

PMID - 36678637

SN - 1999-4923

ER -

BibTex |

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BibTex (up to 50 authors) Copy

@article{2022_Vysochinskaya,

author = {Vera Vysochinskaya and Sergey Mikhailovich Shishlyannikov and Yana A. Zabrodskaya and Elena Shmendel and Sergey Klotchenko and Olga Dobrovolskaya and Nina Gavrilova and Darya Makarova and Marina Plotnikova and Ekaterina Elpaeva and Andrey Gorshkov and Dmitry Moshkoff and Michael Maslov and Andrey Vasin},

title = {Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells},

journal = {Pharmaceutics},

year = {2022},

volume = {15},

publisher = {MDPI},

month = {dec},

url = {https://www.mdpi.com/1999-4923/15/1/8},

number = {1},

pages = {8},

doi = {10.3390/pharmaceutics15010008}

}

MLA

Cite this

MLA Copy

Vysochinskaya, Vera, et al. “Influence of Lipid Composition of Cationic Liposomes 2X3-DOPE on mRNA Delivery into Eukaryotic Cells.” Pharmaceutics, vol. 15, no. 1, Dec. 2022, p. 8. https://www.mdpi.com/1999-4923/15/1/8.

Publisher

MDPI

Journal

Pharmaceutics

scimago Q1

wos Q1

SJR

1.075

CiteScore

10.0

Impact factor

5.5

ISSN

19994923 (Print, Electronic)

Labs

Laboratory of Lipids

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