Open Access
Plants, volume 10, issue 12, pages 2703
Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro
Zayachkovskaya Tatyina
1
,
Domblides Elena
1
,
Zayachkovsky Vladimir
1
,
Kan Lyudmila
1
,
Domblides Arthur
1
,
Soldatenko Alexey
1
Publication type: Journal Article
Publication date: 2021-12-08
Plant Science
Ecology, Evolution, Behavior and Systematics
Ecology
Abstract
The unique and balanced components of the biochemical composition, together with high antioxidant activity, make the red beet necessary a dietary vegetable crop, much contributing to healthy food ration. The application of the technology for producing gynogenic plants in vitro increases the genetic diversity and significantly reduces the period of time required to obtain the appropriate homozygous lines used to create the F1 hybrids that are demanded in the market. For induction of gynogenesis, we used IMB medium developed by us with the addition of 55 g/L sucrose, 3 g/L phytogel, 200 mg/L ampicillin, and 0.4 mg/L thidiazuron (TDZ) and cultured at 28 °C in the dark for 4–6 weeks. Shoot regeneration from embryoids and callus was performed on MS medium with 20 g/L sucrose, 3 g/L phytogel, 1 mg/L 6-benzylaminopurine (BAP), and 0.1 mg/L gibberellic acid (GA3). Immersion of the obtained microshoots with 5–7 well-developed leaves for 10–15 s into concentrated sterile indole-3-butyric acid (IBA) solution (50 mg/L) followed by their cultivation on solid medium ½ IMB with 2% sucrose and 3 g/L phytogel was the most efficient method for root formation. The addition of silver nitrate (22 mg/L) to the nutrient medium provoked an increase in the number of induced ovules up to nine per Petri dish (up to 25% of induced ovules). Gynogenic development was produced in six out of 11 genotypes studied, and the plants that were then acclimatized to ex vitro conditions were obtained in three genotypes (Nezhnost’, Dobrynya, b/a 128). The evaluation of ploidy of gynogenic plants that was carried out by flow cytometry and direct counting of chromosomes stained with propion-lacmoide revealed that all obtained gynogenic plants were haploids (2n = x = 9).
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Zayachkovskaya T. et al. Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro // Plants. 2021. Vol. 10. No. 12. p. 2703.
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Zayachkovskaya T., Domblides E., Zayachkovsky V., Kan L., Domblides A., Soldatenko A. Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro // Plants. 2021. Vol. 10. No. 12. p. 2703.
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TY - JOUR
DO - 10.3390/plants10122703
UR - https://doi.org/10.3390%2Fplants10122703
TI - Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro
T2 - Plants
AU - Zayachkovsky, Vladimir
AU - Kan, Lyudmila
AU - Domblides, Arthur
AU - Soldatenko, Alexey
AU - Zayachkovskaya, Tatyina
AU - Domblides, Elena
PY - 2021
DA - 2021/12/08 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 2703
IS - 12
VL - 10
SN - 2223-7747
ER -
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@article{2021_Zayachkovskaya,
author = {Vladimir Zayachkovsky and Lyudmila Kan and Arthur Domblides and Alexey Soldatenko and Tatyina Zayachkovskaya and Elena Domblides},
title = {Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro},
journal = {Plants},
year = {2021},
volume = {10},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {dec},
url = {https://doi.org/10.3390%2Fplants10122703},
number = {12},
pages = {2703},
doi = {10.3390/plants10122703}
}
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MLA
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Zayachkovskaya, Tatyina, et al. “Production of Gynogenic Plants of Red Beet (Beta vulgaris L.) in Unpollinated Ovule Culture In Vitro.” Plants, vol. 10, no. 12, Dec. 2021, p. 2703. https://doi.org/10.3390%2Fplants10122703.
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