Polymer-Based Conductive Nanocomposites for the Development of Bioanodes Using Membrane-Bound Enzyme Systems of Bacteria Gluconobacter oxydans in Biofuel Cells

The development of biofuel cells (BFCs) currently has high potential since these devices can be used as alternative energy sources. This work studies promising materials for biomaterial immobilization in bioelectrochemical devices based on a comparative analysis of the energy characteristics (generated potential, internal resistance, power) of biofuel cells. Bioanodes are formed by the immobilization of membrane-bound enzyme systems of Gluconobacter oxydans VKM V-1280 bacteria containing pyrroloquinolinquinone-dependent dehydrogenases into hydrogels of polymer-based composites with carbon nanotubes. Natural and synthetic polymers are used as matrices, and multi-walled carbon nanotubes oxidized in hydrogen peroxide vapor (MWCNTox) are used as fillers. The intensity ratio of two characteristic peaks associated with the presence of atoms C in the sp3 and sp2 hybridization for the pristine and oxidized materials is 0.933 and 0.766, respectively. This proves a reduced degree of MWCNTox defectiveness compared to the pristine nanotubes. MWCNTox in the bioanode composites significantly improve the energy characteristics of the BFCs. Chitosan hydrogel in composition with MWCNTox is the most promising material for biocatalyst immobilization for the development of bioelectrochemical systems. The maximum power density was 1.39 × 10−5 W/mm2, which is 2 times higher than the power of BFCs based on other polymer nanocomposites.