In Vivo Anti-Inflammatory and Wound Healing Activity of Extracts and Micro-Aerogels of Bursera microphylla A. Gray

Chitosan micro-aerogels (CsM) are an innovative strategy for the controlled release of healing and anti-inflammatory ingredients. Although Bursera microphylla has anti-inflammatory activity in vitro, its in vivo effect is unknown. This study evaluated the anti-inflammatory and wound-healing effects of extracts and micro-aerogels of B. microphylla. Chitosan micro-aerogels loaded with 0.5% (CsMBT-0.5) and 1% (CsMBT-1) B. microphylla ethanol extract were characterized by SEM, FTIR, TGA, and moisture absorption. Cytotoxicity was assessed by MTT assay, and anti-inflammatory effects in vitro were evaluated by NO quantification. Anti-inflammatory and wound-healing effects in vivo were tested in CD1 mice. The microparticles measured 135–180 μm. FTIR showed that the extract’s compounds remained unchanged during synthesis. TGA indicated degradation of the micro-aerogels between 250–350 °C and reduced moisture absorption when loaded with the extract. The extract inhibited NO release by 36% at 6.25 μg/mL and CsMBT-1 by 46% at 100 μg/mL (p < 0.05). The extract and CsMBT-0.5 in mice reduced ear swelling by 70% at 30 mg/mL (p < 0.0001). The extract reduced wound size by day 9, while CsMBT-0.5 accelerated wound closure from day 1 (p < 0.05), indicating that chitosan micro-aerogels were a promising anti-inflammatory and wound-healing treatment option.