Open Access
Toxins, volume 13, issue 11, pages 746
Bacillus thuringiensis Spores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality
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Department of Biosciences, Faculty of Science and Engineering, Swansea University, Swansea SA2 8PP, Wales, UK
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Publication type: Journal Article
Publication date: 2021-10-21
PubMed ID:
34822531
Health, Toxicology and Mutagenesis
Toxicology
Abstract
The insect integument (exoskeleton) is an effective physiochemical barrier that limits disease-causing agents to a few portals of entry, including the gastrointestinal and reproductive tracts. The bacterial biopesticide Bacillus thuringiensis (Bt) enters the insect host via the mouth and must thwart gut-based defences to make its way into the body cavity (haemocoel) and establish infection. We sought to uncover the main antibacterial defences of the midgut and the pathophysiological features of Bt in a notable insect pest, the Colorado potato beetle Leptinotarsa decemlineata (CPB). Exposing the beetles to both Bt spores and their Cry3A toxins (crystalline δ-endotoxins) via oral inoculation led to higher mortality levels when compared to either spores or Cry3A toxins alone. Within 12 h post-exposure, Cry3A toxins caused a 1.5-fold increase in the levels of reactive oxygen species (ROS) and malondialdehyde (lipid peroxidation) within the midgut – key indicators of tissue damage. When Cry3A toxins are combined with spores, gross redox imbalance and ‘oxidation stress’ is apparent in beetle larvae. The insect detoxification system is activated when Bt spores and Cry3A toxins are administered alone or in combination to mitigate toxicosis, in addition to elevated mRNA levels of candidate defence genes (pattern-recognition receptor, stress-regulation, serine proteases, and prosaposin-like protein). The presence of bacterial spores and/or Cry3A toxins coincides with subtle changes in microbial community composition of the midgut, such as decreased Pseudomonas abundance at 48 h post inoculation. Both Bt spores and Cry3A toxins have negative impacts on larval health, and when combined, likely cause metabolic derangement, due to multiple tissue targets being compromised.
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Citations by publishers
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3
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 37.5%
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Springer Nature
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Springer Nature
2 publications, 25%
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Elsevier
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1 publication, 12.5%
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Frontiers Media S.A.
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Frontiers Media S.A.
1 publication, 12.5%
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Wiley
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Wiley
1 publication, 12.5%
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Dubovskiy I. M. et al. Bacillus thuringiensis Spores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality // Toxins. 2021. Vol. 13. No. 11. p. 746.
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Dubovskiy I. M., Grizanova E. V., Tereshchenko D., Krytsyna T. I., Alikina T., Kalmykova G., Kabilov M. R., Coates C. Bacillus thuringiensis Spores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality // Toxins. 2021. Vol. 13. No. 11. p. 746.
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TY - JOUR
DO - 10.3390/toxins13110746
UR - https://doi.org/10.3390%2Ftoxins13110746
TI - Bacillus thuringiensis Spores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality
T2 - Toxins
AU - Tereshchenko, Daria
AU - Krytsyna, Tatiana I
AU - Alikina, Tatyana
AU - Kalmykova, Galina
AU - Dubovskiy, Ivan M.
AU - Grizanova, Ekaterina V
AU - Kabilov, Marsel R.
AU - Coates, Christopher
PY - 2021
DA - 2021/10/21 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 746
IS - 11
VL - 13
PMID - 34822531
SN - 2072-6651
ER -
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@article{2021_Dubovskiy,
author = {Daria Tereshchenko and Tatiana I Krytsyna and Tatyana Alikina and Galina Kalmykova and Ivan M. Dubovskiy and Ekaterina V Grizanova and Marsel R. Kabilov and Christopher Coates},
title = {Bacillus thuringiensis Spores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality},
journal = {Toxins},
year = {2021},
volume = {13},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {oct},
url = {https://doi.org/10.3390%2Ftoxins13110746},
number = {11},
pages = {746},
doi = {10.3390/toxins13110746}
}
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MLA
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Dubovskiy, Ivan M., et al. “Bacillus thuringiensis Spores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality.” Toxins, vol. 13, no. 11, Oct. 2021, p. 746. https://doi.org/10.3390%2Ftoxins13110746.