Open Access
Vaccines, volume 12, issue 2, pages 129
Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies
Pavel P Solodkov
1
,
Alexander M. Najakshin
1
,
Nikolai A Chikaev
1
,
Sergey V Kulemzin
1
,
Ludmila V. Mechetina
1
,
Konstantin Baranov
1
,
Sergey V Guselnikov
1
,
Tatyana Belovezhets
1
,
Anton N. Chikaev
1
,
Olga Y Volkova
1
,
Alexander G Markhaev
2
,
Yulia V Kononova
2
,
Alexander Yu Alekseev
2, 3
,
Marina A Gulyaeva
2, 3
,
Alexander M. Shestopalov
2, 3
,
Publication type: Journal Article
Publication date: 2024-01-26
Drug Discovery
Pharmacology
Infectious Diseases
Pharmacology (medical)
Immunology
Abstract
The emergence of SARS-CoV-2 mutant variants has posed a significant challenge to both the prevention and treatment of COVID-19 with anti-coronaviral neutralizing antibodies. The latest viral variants demonstrate pronounced resistance to the vast majority of human monoclonal antibodies raised against the ancestral Wuhan variant. Less is known about the susceptibility of the evolved virus to camelid nanobodies developed at the start of the pandemic. In this study, we compared nanobody repertoires raised in the same llama after immunization with Wuhan’s RBD variant and after subsequent serial immunization with a variety of RBD variants, including that of SARS-CoV-1. We show that initial immunization induced highly potent nanobodies, which efficiently protected Syrian hamsters from infection with the ancestral Wuhan virus. These nanobodies, however, mostly lacked the activity against SARS-CoV-2 omicron-pseudotyped viruses. In contrast, serial immunization with different RBD variants resulted in the generation of nanobodies demonstrating a higher degree of somatic mutagenesis and a broad range of neutralization. Four nanobodies recognizing distinct epitopes were shown to potently neutralize a spectrum of omicron variants, including those of the XBB sublineage. Our data show that nanobodies broadly neutralizing SARS-CoV-2 variants may be readily induced by a serial variant RBD immunization.
Top-30
Citations by journals
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Frontiers in Immunology
1 publication, 50%
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Russian Chemical Reviews
1 publication, 50%
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Citations by publishers
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Frontiers Media S.A.
1 publication, 50%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 50%
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Solodkov P. P. et al. Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies // Vaccines. 2024. Vol. 12. No. 2. p. 129.
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Solodkov P. P., Najakshin A. M., Chikaev N. A., Kulemzin S. V., Mechetina L. V., Baranov K., Guselnikov S. V., Gorchakov A. A., Belovezhets T., Chikaev A. N., Volkova O. Y., Markhaev A. G., Kononova Y. V., Alekseev A. Yu., Gulyaeva M. A., Shestopalov A. M., Taranin A. Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies // Vaccines. 2024. Vol. 12. No. 2. p. 129.
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TY - JOUR
DO - 10.3390/vaccines12020129
UR - https://doi.org/10.3390/vaccines12020129
TI - Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies
T2 - Vaccines
AU - Solodkov, Pavel P
AU - Najakshin, Alexander M.
AU - Chikaev, Nikolai A
AU - Kulemzin, Sergey V
AU - Mechetina, Ludmila V.
AU - Baranov, Konstantin
AU - Guselnikov, Sergey V
AU - Gorchakov, Andrey A
AU - Belovezhets, Tatyana
AU - Chikaev, Anton N.
AU - Volkova, Olga Y
AU - Markhaev, Alexander G
AU - Kononova, Yulia V
AU - Alekseev, Alexander Yu
AU - Gulyaeva, Marina A
AU - Shestopalov, Alexander M.
AU - Taranin, Alexander
PY - 2024
DA - 2024/01/26 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 129
IS - 2
VL - 12
SN - 2076-393X
SN - 2076-393X
ER -
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@article{2024_Solodkov,
author = {Pavel P Solodkov and Alexander M. Najakshin and Nikolai A Chikaev and Sergey V Kulemzin and Ludmila V. Mechetina and Konstantin Baranov and Sergey V Guselnikov and Andrey A Gorchakov and Tatyana Belovezhets and Anton N. Chikaev and Olga Y Volkova and Alexander G Markhaev and Yulia V Kononova and Alexander Yu Alekseev and Marina A Gulyaeva and Alexander M. Shestopalov and Alexander Taranin},
title = {Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies},
journal = {Vaccines},
year = {2024},
volume = {12},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jan},
url = {https://doi.org/10.3390/vaccines12020129},
number = {2},
pages = {129},
doi = {10.3390/vaccines12020129}
}
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Solodkov, Pavel P., et al. “Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies.” Vaccines, vol. 12, no. 2, Jan. 2024, p. 129. https://doi.org/10.3390/vaccines12020129.