Open Access
Viruses, volume 13, issue 6, pages 1133
Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera
Publication type: Journal Article
Publication date: 2021-06-12
PubMed ID:
34204732
Infectious Diseases
Virology
Abstract
The COVID-19 pandemic caused by SARS-CoV-2 has posed a global threat to human lives and economics. One of the best ways to determine protection against the infection is to quantify the neutralizing activity of serum antibodies. Multiple assays have been developed to validate SARS-CoV-2 neutralization; most of them utilized lentiviral or vesicular stomatitis virus-based particles pseudotyped with the spike (S) protein, making them safe and acceptable to work with in many labs. However, these systems are only capable of measuring infection with purified particles. This study has developed a pseudoviral assay with replication-dependent reporter vectors that can accurately quantify the level of infection directly from the virus producing cell to the permissive target cell. Comparative analysis of cell-free and cell-to-cell infection revealed that the neutralizing activity of convalescent sera was more than tenfold lower in cell cocultures than in the cell-free mode of infection. As the pseudoviral system could not properly model the mechanisms of SARS-CoV-2 transmission, similar experiments were performed with replication-competent coronavirus, which detected nearly complete SARS-CoV-2 cell-to-cell infection resistance to neutralization by convalescent sera. These findings suggest that the cell-to-cell mode of SARS-CoV-2 transmission, for which the mechanisms are largely unknown, could be of great importance for treatment and prevention of COVID-19.
Top-30
Citations by journals
|
1
2
3
4
|
|
|
Viruses
4 publications, 17.39%
|
|
|
Cellular and Molecular Life Sciences
1 publication, 4.35%
|
|
|
Molecular Biology
1 publication, 4.35%
|
|
|
International Journal of Molecular Sciences
1 publication, 4.35%
|
|
|
Acta Naturae
1 publication, 4.35%
|
|
|
Science Translational Medicine
1 publication, 4.35%
|
|
|
Voprosy Virusologii
1 publication, 4.35%
|
|
|
Journal of Biomedical Science
1 publication, 4.35%
|
|
|
Frontiers in Immunology
1 publication, 4.35%
|
|
|
PLoS Pathogens
1 publication, 4.35%
|
|
|
mBio
1 publication, 4.35%
|
|
|
Doklady Biochemistry and Biophysics
1 publication, 4.35%
|
|
|
PLoS Biology
1 publication, 4.35%
|
|
|
Vaccines
1 publication, 4.35%
|
|
|
Biomolecules
1 publication, 4.35%
|
|
|
Vrach
1 publication, 4.35%
|
|
|
Доклады Российской академии наук Науки о жизни
1 publication, 4.35%
|
|
|
Russian Chemical Reviews
1 publication, 4.35%
|
|
|
1
2
3
4
|
Citations by publishers
|
1
2
3
4
5
6
7
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
7 publications, 30.43%
|
|
|
Springer Nature
2 publications, 8.7%
|
|
|
Pleiades Publishing
2 publications, 8.7%
|
|
|
Public Library of Science (PLoS)
2 publications, 8.7%
|
|
|
Acta Naturae Ltd
1 publication, 4.35%
|
|
|
American Association for the Advancement of Science (AAAS)
1 publication, 4.35%
|
|
|
Cold Spring Harbor Laboratory
1 publication, 4.35%
|
|
|
Central Research Institute for Epidemiology
1 publication, 4.35%
|
|
|
Frontiers Media S.A.
1 publication, 4.35%
|
|
|
American Society for Microbiology
1 publication, 4.35%
|
|
|
Russkiy Vrach Publishing House
1 publication, 4.35%
|
|
|
The Russian Academy of Sciences
1 publication, 4.35%
|
|
|
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 4.35%
|
|
|
1
2
3
4
5
6
7
|
- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2021,2022,2023,2024],"ids":[0,0,0,0],"codes":[0,0,0,0],"imageUrls":["","","",""],"datasets":[{"label":"Citations number","data":[3,5,7,8],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["13.04","21.74","30.43","34.78"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Viruses","Cellular and Molecular Life Sciences","Molecular Biology","International Journal of Molecular Sciences","Acta Naturae","Science Translational Medicine","Voprosy Virusologii","Journal of Biomedical Science","Frontiers in Immunology","PLoS Pathogens","mBio","Doklady Biochemistry and Biophysics","PLoS Biology","Vaccines","Biomolecules","Vrach","\u0414\u043e\u043a\u043b\u0430\u0434\u044b \u0420\u043e\u0441\u0441\u0438\u0439\u0441\u043a\u043e\u0439 \u0430\u043a\u0430\u0434\u0435\u043c\u0438\u0438 \u043d\u0430\u0443\u043a \u041d\u0430\u0443\u043a\u0438 \u043e \u0436\u0438\u0437\u043d\u0438","Russian Chemical Reviews"],"ids":[2455,5151,1134,14627,7401,358,6684,24316,1699,5093,9121,3773,13133,24868,13780,27497,37596,23802],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/HxgFXojL9GSWD86Qe827ZjqG8ciNkkviz42FIGJU_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/2shicgr9eTVtQfz3pWfNIr2VLO1YBjXcuQ2dkj5c_medium.webp","\/storage\/images\/resized\/BMSCJq2BB4zMd1MEqeBIksqeh6Nq2w1sKUe3W6QR_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp"],"datasets":[{"label":"","data":[4,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[17.39,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature","Pleiades Publishing","Public Library of Science (PLoS)","Acta Naturae Ltd","American Association for the Advancement of Science (AAAS)","Cold Spring Harbor Laboratory","Central Research Institute for Epidemiology","Frontiers Media S.A.","American Society for Microbiology","Russkiy Vrach Publishing House","The Russian Academy of Sciences","Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii"],"ids":[202,8,101,344,7905,189,6909,7217,208,142,6963,7019,9422],"codes":[0,0,0,0,0,0,0,0,0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp","\/storage\/images\/resized\/oZgeErrVFhuDksyqFURLvYS1wtVSBWczh001igGo_medium.webp","\/storage\/images\/resized\/PRGhlgB4OKRltSNtT39eA6wlnOTGRap1QQ6FQHih_medium.webp","","\/storage\/images\/resized\/s10mcsCV4OAUg9O2KrqOquQC0PhyLMI8hUUkuflM_medium.webp","\/storage\/images\/resized\/hg4jJjT8wVGtHstBCc0zk465Mg9pLx3G4odCDOqE_medium.webp","\/storage\/images\/resized\/HxgFXojL9GSWD86Qe827ZjqG8ciNkkviz42FIGJU_medium.webp","\/storage\/images\/resized\/4QWA67eqfcfyOiA8Wk7YnqroHFqQbTsmDJUYTCTg_medium.webp","\/storage\/images\/resized\/VPE7q24PXoHr5SS2SqfEgxccSMUxWLfZINOX60uo_medium.webp","\/storage\/images\/resized\/2shicgr9eTVtQfz3pWfNIr2VLO1YBjXcuQ2dkj5c_medium.webp","\/storage\/images\/resized\/BMSCJq2BB4zMd1MEqeBIksqeh6Nq2w1sKUe3W6QR_medium.webp","\/storage\/images\/resized\/9Mus3KG1Tkd7Bwaurt8H3RwWh0CxRlGoO6ng9UK1_medium.webp"],"datasets":[{"label":"","data":[7,2,2,2,1,1,1,1,1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[30.43,8.7,8.7,8.7,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35,4.35],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":false}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartiles":{"type":"bar","data":{"show":true,"labels":[2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,0,2,0],"percentage":["0","0","8.7","0"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,1,0,1],"percentage":["0","4.35","0","4.35"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[0,0,0,0],"percentage":["0","0","0","0"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[2,4,4,5],"percentage":["8.7","17.39","17.39","21.74"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[1,0,1,2],"percentage":["4.35","0","4.35","8.7"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by SCImago per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"yearsCitationsQuartilesWs":{"type":"bar","data":{"show":true,"labels":[2021,2022,2023,2024],"ids":[],"codes":[],"imageUrls":[],"datasets":[{"label":"Q4","backgroundColor":"rgb(221,90,78)","data":[0,1,1,1],"percentage":["0","4.35","4.35","4.35"]},{"label":"Q3","backgroundColor":"rgb(251, 163,83)","data":[0,0,0,0],"percentage":["0","0","0","0"]},{"label":"Q2","backgroundColor":"rgb(232, 213, 89)","data":[1,3,0,0],"percentage":["4.35","13.04","0","0"]},{"label":"Q1","backgroundColor":"rgb(164, 207, 99)","data":[1,1,4,5],"percentage":["4.35","4.35","17.39","21.74"]},{"label":"Quartile not defined","backgroundColor":"#E5E7EB","data":[1,0,2,2],"percentage":["4.35","0","8.7","8.7"]}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"},"stacked":true}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations quartiles by WoS per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Kruglova N. S. et al. Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera // Viruses. 2021. Vol. 13. No. 6. p. 1133.
GOST all authors (up to 50)
Copy
Kruglova N. S., Siniavin A., Гущин В. А., Mazurov D. Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera // Viruses. 2021. Vol. 13. No. 6. p. 1133.
Cite this
RIS
Copy
TY - JOUR
DO - 10.3390/v13061133
UR - https://doi.org/10.3390/v13061133
TI - Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera
T2 - Viruses
AU - Mazurov, Dmitriy
AU - Kruglova, Natal’ya S.
AU - Siniavin, Andrei
AU - Гущин, В. А.
PY - 2021
DA - 2021/06/12 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 1133
IS - 6
VL - 13
PMID - 34204732
SN - 1999-4915
SN - 1999-4915
ER -
Cite this
BibTex
Copy
@article{2021_Kruglova,
author = {Dmitriy Mazurov and Natal’ya S. Kruglova and Andrei Siniavin and В. А. Гущин},
title = {Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera},
journal = {Viruses},
year = {2021},
volume = {13},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jun},
url = {https://doi.org/10.3390/v13061133},
number = {6},
pages = {1133},
doi = {10.3390/v13061133}
}
Cite this
MLA
Copy
Kruglova, Natal’ya S., et al. “Different Neutralization Sensitivity of SARS-CoV-2 Cell-to-Cell and Cell-Free Modes of Infection to Convalescent Sera.” Viruses, vol. 13, no. 6, Jun. 2021, p. 1133. https://doi.org/10.3390/v13061133.