Open Access

Antioxidants, volume 9, issue 5, pages 377

The delayed neuroprotective effect of methylene blue in experimental rat brain trauma

Genrikhs Elizaveta E. ¹

Stelmashook Elena V. ¹

Voronkov Dmitriy N ¹

Novikova Svetlana V ¹

Alexandrova Olga P ¹

Gulyaev Mikhail ²

Isaev Nickolay K. ^{1, 3}

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Research Center of Neurology, Volokolamskoe shosse 80, 125367 Moscow, Russia |

Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, 119991 Moscow, Russia |

Biological Faculty, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia |

Publication type: Journal Article

Publication date: 2020-05-02

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Antioxidants

Quartile SCImago

Quartile WOS

Impact factor: 7

ISSN: 20763921

DOI: 10.3390/antiox9050377

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PubMed ID: 32370131

Biochemistry

Molecular Biology

Cell Biology

Clinical Biochemistry

Physiology

Abstract

After traumatic brain injury (TBI), an increase in dysfunction of the limbs contralateral to injury focus was observed. Using different behavioral tests, we found that a single intravenous injection of methylene blue (MB, 1 mg/kg) 30 min after the injury reduced the impairment of the motor functions of the limbs from 7 to 120 days after TBI. Administration of methylene blue 30 min after the injury and then monthly (six injections in total) was the most effective both in terms of preservation of limb function and duration of therapeutic action. This therapeutic effect was clearly manifested from the seventh day and continued until the end of the experiment—by the 180th day after TBI. MB is known to possess antioxidant properties; it has a protective effect against TBI by promoting autophagy and minimizing lesion volume in the first two weeks after TBI. Studies of the brains on the 180th day after TBI demonstrated that the monthly treatment of animals with MB statistically significantly prevented an increase in the density of microglial cells in the ipsilateral hemisphere and a decrease in the thickness of the corpus callosum in the contralateral hemisphere in comparison with untreated animals. However, on the 180th day after TBI, the magnetic resonance imaging scan of the animal brains did not show a significant reduction in the volume of the lesion in MB-treated animals. These findings are important for understanding the development of the long-term effects of TBI and expand the required therapeutic window for targeted neuroprotective interventions.

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	1
Brain Research	Brain Research, 1, 33.33% Brain Research 1 publication, 33.33%
Biochemistry (Moscow)	Biochemistry (Moscow), 1, 33.33% Biochemistry (Moscow) 1 publication, 33.33%
Reviews in the Neurosciences	Reviews in the Neurosciences, 1, 33.33% Reviews in the Neurosciences 1 publication, 33.33%
	1

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	1
Elsevier	Elsevier, 1, 33.33% Elsevier 1 publication, 33.33%
Pleiades Publishing	Pleiades Publishing, 1, 33.33% Pleiades Publishing 1 publication, 33.33%
Walter de Gruyter	Walter de Gruyter, 1, 33.33% Walter de Gruyter 1 publication, 33.33%
	1

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Genrikhs E. E. et al. The delayed neuroprotective effect of methylene blue in experimental rat brain trauma // Antioxidants. 2020. Vol. 9. No. 5. p. 377.

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Genrikhs E. E., Stelmashook E. V., Voronkov D. N., Novikova S. V., Alexandrova O. P., Gulyaev M., Isaev N. K. The delayed neuroprotective effect of methylene blue in experimental rat brain trauma // Antioxidants. 2020. Vol. 9. No. 5. p. 377.

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TY - JOUR

DO - 10.3390/antiox9050377

UR - https://doi.org/10.3390%2Fantiox9050377

TI - The delayed neuroprotective effect of methylene blue in experimental rat brain trauma

T2 - Antioxidants

AU - Genrikhs, Elizaveta E.

AU - Stelmashook, Elena V.

AU - Voronkov, Dmitriy N

AU - Novikova, Svetlana V

AU - Alexandrova, Olga P

AU - Isaev, Nickolay K.

AU - Gulyaev, Mikhail

PY - 2020

DA - 2020/05/02 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 377

IS - 5

VL - 9

PMID - 32370131

SN - 2076-3921

ER -

BibTex |

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@article{2020_Genrikhs,

author = {Elizaveta E. Genrikhs and Elena V. Stelmashook and Dmitriy N Voronkov and Svetlana V Novikova and Olga P Alexandrova and Nickolay K. Isaev and Mikhail Gulyaev},

title = {The delayed neuroprotective effect of methylene blue in experimental rat brain trauma},

journal = {Antioxidants},

year = {2020},

volume = {9},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {may},

url = {https://doi.org/10.3390%2Fantiox9050377},

number = {5},

pages = {377},

doi = {10.3390/antiox9050377}

}

MLA

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Genrikhs, Elizaveta E., et al. “The delayed neuroprotective effect of methylene blue in experimental rat brain trauma.” Antioxidants, vol. 9, no. 5, May. 2020, p. 377. https://doi.org/10.3390%2Fantiox9050377.

Found error?

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Antioxidants

Quartile SCImago

Quartile WOS

Impact factor

ISSN

20763921 (Electronic)

Profiles

Gulyaev, Mikhail Vladimirovich