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Biosensors, volume 12, issue 9, pages 768

Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode

Samodelova Mariia V ¹

Kapitanova Olesya O. ^{1, 2}

Meshcheryakova Nadezda F ¹

Novikov Sergey ²

Yarenkov Nikita R ¹

Streletskii R A ³

Yakubovsky Dmitry I. ²

Grabovenko F I ¹

Zhdanov Gleb A ¹

Arsenin Aleksey ²

Volkov Valentyn S. ²

Zavyalova Elena ¹

Veselova Irina A. ¹

Zvereva Maria ¹

Hide authors affiliations Show authors affiliations: 3 affiliations

Faculty of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia |

Center for Photonics and 2D Materials, Moscow Institute of Physics and Technology, 141700 Dolgoprudny, Russia |

Faculty of Physics, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia |

Publication type: Journal Article

Publication date: 2022-09-19

Multidisciplinary Digital Publishing Institute (MDPI)

Journal: Biosensors

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Quartile WOS

Impact factor: 5.4

ISSN: 20796374, 0265928X

DOI: 10.3390/bios12090768

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General Medicine

Clinical Biochemistry

Analytical Chemistry

Biotechnology

Instrumentation

Biomedical Engineering

Engineering (miscellaneous)

Abstract

The recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has posed a great challenge for the development of ultra-fast methods for virus identification based on sensor principles. We created a structure modeling surface and size of the SARS-CoV-2 virus and used it in comparison with the standard antigen SARS-CoV-2—the receptor-binding domain (RBD) of the S-protein of the envelope of the SARS-CoV-2 virus from the Wuhan strain—for the development of detection of coronaviruses using a DNA-modified, surface-enhanced Raman scattering (SERS)-based aptasensor in sandwich mode: a primary aptamer attached to the plasmonic surface—RBD-covered Ag nanoparticle—the Cy3-labeled secondary aptamer. Fabricated novel hybrid plasmonic structures based on “Ag mirror-SiO2-nanostructured Ag” demonstrate sensitivity for the detection of investigated analytes due to the combination of localized surface plasmons in nanostructured silver surface and the gap surface plasmons in a thin dielectric layer of SiO2 between silver layers. A specific SERS signal has been obtained from SERS-active compounds with RBD-specific DNA aptamers that selectively bind to the S protein of synthetic virion (dissociation constants of DNA-aptamer complexes with protein in the range of 10 nM). The purpose of the study is to systematically analyze the combination of components in an aptamer-based sandwich system. A developed virus size simulating silver particles adsorbed on an aptamer-coated sensor provided a signal different from free RBD. The data obtained are consistent with the theory of signal amplification depending on the distance of the active compound from the amplifying surface and the nature of such a compound. The ability to detect the target virus due to specific interaction with such DNA is quantitatively controlled by the degree of the quenching SERS signal from the labeled compound. Developed indicator sandwich-type systems demonstrate high stability. Such a platform does not require special permissions to work with viruses. Therefore, our approach creates the promising basis for fostering the practical application of ultra-fast, amplification-free methods for detecting coronaviruses based on SARS-CoV-2.

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	1
Materials	Materials, 1, 33.33% Materials 1 publication, 33.33%
Biosensors	Biosensors, 1, 33.33% Biosensors 1 publication, 33.33%
Optical Materials	Optical Materials, 1, 33.33% Optical Materials 1 publication, 33.33%
	1

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	1 2
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 2, 66.67% Multidisciplinary Digital Publishing Institute (MDPI) 2 publications, 66.67%
Elsevier	Elsevier, 1, 33.33% Elsevier 1 publication, 33.33%
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Samodelova M. V. et al. Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode // Biosensors. 2022. Vol. 12. No. 9. p. 768.

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Samodelova M. V., Kapitanova O. O., Meshcheryakova N. F., Novikov S., Yarenkov N. R., Streletskii R. A., Yakubovsky D. I., Grabovenko F. I., Zhdanov G. A., Arsenin A., Volkov V. S., Zavyalova E., Veselova I. A., Zvereva M. Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode // Biosensors. 2022. Vol. 12. No. 9. p. 768.

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TY - JOUR

DO - 10.3390/bios12090768

UR - https://doi.org/10.3390%2Fbios12090768

TI - Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode

T2 - Biosensors

AU - Samodelova, Mariia V

AU - Kapitanova, Olesya O.

AU - Yakubovsky, Dmitry I.

AU - Meshcheryakova, Nadezda F

AU - Yarenkov, Nikita R

AU - Veselova, Irina A.

AU - Novikov, Sergey

AU - Streletskii, R A

AU - Grabovenko, F I

AU - Zhdanov, Gleb A

AU - Arsenin, Aleksey

AU - Volkov, Valentyn S.

AU - Zavyalova, Elena

AU - Zvereva, Maria

PY - 2022

DA - 2022/09/19 00:00:00

PB - Multidisciplinary Digital Publishing Institute (MDPI)

SP - 768

IS - 9

VL - 12

SN - 2079-6374

SN - 0265-928X

ER -

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@article{2022_Samodelova,

author = {Mariia V Samodelova and Olesya O. Kapitanova and Dmitry I. Yakubovsky and Nadezda F Meshcheryakova and Nikita R Yarenkov and Irina A. Veselova and Sergey Novikov and R A Streletskii and F I Grabovenko and Gleb A Zhdanov and Aleksey Arsenin and Valentyn S. Volkov and Elena Zavyalova and Maria Zvereva},

title = {Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode},

journal = {Biosensors},

year = {2022},

volume = {12},

publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},

month = {sep},

url = {https://doi.org/10.3390%2Fbios12090768},

number = {9},

pages = {768},

doi = {10.3390/bios12090768}

}

MLA

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Samodelova, Mariia V., et al. “Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode.” Biosensors, vol. 12, no. 9, Sep. 2022, p. 768. https://doi.org/10.3390%2Fbios12090768.

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Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

Journal

Biosensors

Quartile SCImago

Quartile WOS

Impact factor

5.4

ISSN

20796374 (Electronic)
0265928X (Print)

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