Open Access

Biosensors

, volume 9 , issue 3 , pages 84

Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera

Dan Tao ^{1, 2}

Bingqing Shui ¹

Yingying Gu ¹

Jing Cheng ¹

Weiying Zhang ³

N. Jaffrezic-Renault ⁴

Shizhen Song ²

Zhenzhong Guo ¹

Hide authors affiliations Show authors affiliations: 4 affiliations

Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China |

Resources and Environmental Engineering College, Wuhan University of Science and Technology, Wuhan 430081, China |

Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Institute for Interdisciplinary Research, Jianghan University, Wuhan 430056, China |

⁴

University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France |

Publication type: Journal Article

Publication date: 2019-06-30

MDPI

Biosensors

scimago Q1

wos Q1

SJR: 0.885

CiteScore: 9.8

Impact factor: 5.6

ISSN: 20796374, 0265928X

DOI: 10.3390/bios9030084

Copy DOI

PubMed ID: 31262001

General Medicine

Clinical Biochemistry

Abstract

The electrochemical aptamer sensor has been designed for detecting tau381, a critical biomarker of Alzheimer′s disease in human serum. The aptasensor is obtained by immobilizing the aptamer on a carboxyl graphene/thionin/gold nanoparticle modified glassy-carbon electrode. As a probe and bridge molecule, thionin connected carboxyl graphene and gold nanoparticles, and gave the electrical signal. Under optimal conditions, the increment of differential pulse voltammetry signal increased linearly with the logarithm of tau381 concentration in the range from 1.0 pM to 100 pM, and limit of detection was 0.70 pM. The aptasensor reliability was evaluated by determining its selectivity, reproducibility, stability, detection limit, and recovery. Performance analysis of the tau381 aptasensor in 10 patients’ serum samples showed that the aptasensor could screen patients with and without Alzheimer′s disease. The proposed aptasensor has potential for use in clinically diagnosing Alzheimer′s disease in the early stage.

Found

2 citations

Jaffrezic-Renault Nicole

207 publications, 3 704 citations

h-index: 34

Université Marie et Louis Pasteur

Université Claude Bernard Lyon 1

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Tao D. et al. Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera // Biosensors. 2019. Vol. 9. No. 3. p. 84.

GOST all authors (up to 50) Copy

Tao D., Shui B., Gu Y., Cheng J., Zhang W., Jaffrezic-Renault N., Song S., Guo Z. Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera // Biosensors. 2019. Vol. 9. No. 3. p. 84.

RIS |

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RIS Copy

TY - JOUR

DO - 10.3390/bios9030084

UR - https://www.mdpi.com/2079-6374/9/3/84

TI - Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera

T2 - Biosensors

AU - Tao, Dan

AU - Shui, Bingqing

AU - Gu, Yingying

AU - Cheng, Jing

AU - Zhang, Weiying

AU - Jaffrezic-Renault, N.

AU - Song, Shizhen

AU - Guo, Zhenzhong

PY - 2019

DA - 2019/06/30

PB - MDPI

SP - 84

IS - 3

VL - 9

PMID - 31262001

SN - 2079-6374

SN - 0265-928X

ER -

BibTex |

Cite this

BibTex (up to 50 authors) Copy

@article{2019_Tao,

author = {Dan Tao and Bingqing Shui and Yingying Gu and Jing Cheng and Weiying Zhang and N. Jaffrezic-Renault and Shizhen Song and Zhenzhong Guo},

title = {Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera},

journal = {Biosensors},

year = {2019},

volume = {9},

publisher = {MDPI},

month = {jun},

url = {https://www.mdpi.com/2079-6374/9/3/84},

number = {3},

pages = {84},

doi = {10.3390/bios9030084}

}

MLA

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MLA Copy

Tao, Dan, et al. “Development of a Label-Free Electrochemical Aptasensor for the Detection of Tau381 and its Preliminary Application in AD and Non-AD Patients’ Sera.” Biosensors, vol. 9, no. 3, Jun. 2019, p. 84. https://www.mdpi.com/2079-6374/9/3/84.

Publisher

MDPI

Journal

Biosensors

scimago Q1

wos Q1

SJR

0.885

CiteScore

9.8

Impact factor

5.6

ISSN

20796374 (Print, Electronic)

0265928X (Print)

Profiles

Nicole Jaffrezic Jaffrezic-Renault