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Cancers

, том 11 , издание 3 , страницы 351

Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

Galina Zamay ^{1, 2}

Olga Kolovskaya ^{1, 2}

Tatiana Ivanchenko ¹

Tatiana Zamay ^{1, 2}

Dmitry Veprintsev ¹

Valentina Grigorieva ²

Irina Garanzha ²

Alexey Krat ^{2, 3}

Yury Glazyrin ^{1, 2}

Ana Gargaun ⁴

Ivan Lapin ⁵

Valery Svetlichnyi ⁵

Maxim Berezovski ⁴

Anna Kichkailo ^{1, 2}

Скрыть аффилиации авторов Показать аффилиации авторов: 5 аффилиаций

Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Science”, Krasnoyarsk 660036, Russia |

Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenecki, Krasnoyarsk 660022, Russia |

Krasnoyarsk Regional Clinical Cancer Center named after A.I. Kryzhanovsky, Krasnoyarsk 660133, Russia |

⁴

Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, ON K1N 6N5, Canada |

⁵

Laboratory of Advanced Materials and Technology, Siberian Physical-Technical Institute of Tomsk State University, Tomsk 634050, Russia |

Тип публикации: Journal Article

Дата публикации: 2019-03-12

MDPI

Cancers

scimago Q1

wos Q2

БС1

SJR: 1.462

CiteScore: 8.8

Impact factor: 4.4

ISSN: 20726694

DOI: 10.3390/cancers11030351

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PubMed ID: 30871104

Cancer Research

Oncology

Краткое описание

We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.

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Zamay G. et al. Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood // Cancers. 2019. Vol. 11. No. 3. p. 351.

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Zamay G., Kolovskaya O., Ivanchenko T., Zamay T., Veprintsev D., Grigorieva V., Garanzha I., Krat A., Glazyrin Y., Gargaun A., Lapin I., Svetlichnyi V., Berezovski M., Kichkailo A. Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood // Cancers. 2019. Vol. 11. No. 3. p. 351.

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TY - JOUR

DO - 10.3390/cancers11030351

UR - https://doi.org/10.3390/cancers11030351

TI - Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

T2 - Cancers

AU - Zamay, Galina

AU - Kolovskaya, Olga

AU - Ivanchenko, Tatiana

AU - Zamay, Tatiana

AU - Veprintsev, Dmitry

AU - Grigorieva, Valentina

AU - Garanzha, Irina

AU - Krat, Alexey

AU - Glazyrin, Yury

AU - Gargaun, Ana

AU - Lapin, Ivan

AU - Svetlichnyi, Valery

AU - Berezovski, Maxim

AU - Kichkailo, Anna

PY - 2019

DA - 2019/03/12

PB - MDPI

SP - 351

IS - 3

VL - 11

PMID - 30871104

SN - 2072-6694

ER -

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@article{2019_Zamay,

author = {Galina Zamay and Olga Kolovskaya and Tatiana Ivanchenko and Tatiana Zamay and Dmitry Veprintsev and Valentina Grigorieva and Irina Garanzha and Alexey Krat and Yury Glazyrin and Ana Gargaun and Ivan Lapin and Valery Svetlichnyi and Maxim Berezovski and Anna Kichkailo},

title = {Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood},

journal = {Cancers},

year = {2019},

volume = {11},

publisher = {MDPI},

month = {mar},

url = {https://doi.org/10.3390/cancers11030351},

number = {3},

pages = {351},

doi = {10.3390/cancers11030351}

}

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Zamay, Galina, et al. “Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood.” Cancers, vol. 11, no. 3, Mar. 2019, p. 351. https://doi.org/10.3390/cancers11030351.