Open Access
Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood
Galina Zamay
1, 2
,
Olga Kolovskaya
1, 2
,
Tatiana Ivanchenko
1
,
Tatiana Zamay
1, 2
,
Dmitry Veprintsev
1
,
Valentina Grigorieva
2
,
Irina Garanzha
2
,
Alexey Krat
2, 3
,
Yury Glazyrin
1, 2
,
Ana Gargaun
4
,
Ivan Lapin
5
,
Valery Svetlichnyi
5
,
Maxim Berezovski
4
,
Anna Kichkailo
1, 2
3
Krasnoyarsk Regional Clinical Cancer Center named after A.I. Kryzhanovsky, Krasnoyarsk 660133, Russia
|
4
Publication type: Journal Article
Publication date: 2019-03-12
PubMed ID:
30871104
Cancer Research
Oncology
Abstract
We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.
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Total citations:
21
Citations from 2024:
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(14%)
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MLA
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GOST
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Zamay G. et al. Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood // Cancers. 2019. Vol. 11. No. 3. p. 351.
GOST all authors (up to 50)
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Zamay G., Kolovskaya O., Ivanchenko T., Zamay T., Veprintsev D., Grigorieva V., Garanzha I., Krat A., Glazyrin Y., Gargaun A., Lapin I., Svetlichnyi V., Berezovski M., Kichkailo A. Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood // Cancers. 2019. Vol. 11. No. 3. p. 351.
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RIS
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TY - JOUR
DO - 10.3390/cancers11030351
UR - https://doi.org/10.3390/cancers11030351
TI - Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood
T2 - Cancers
AU - Zamay, Galina
AU - Kolovskaya, Olga
AU - Ivanchenko, Tatiana
AU - Zamay, Tatiana
AU - Veprintsev, Dmitry
AU - Grigorieva, Valentina
AU - Garanzha, Irina
AU - Krat, Alexey
AU - Glazyrin, Yury
AU - Gargaun, Ana
AU - Lapin, Ivan
AU - Svetlichnyi, Valery
AU - Berezovski, Maxim
AU - Kichkailo, Anna
PY - 2019
DA - 2019/03/12
PB - MDPI
SP - 351
IS - 3
VL - 11
PMID - 30871104
SN - 2072-6694
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2019_Zamay,
author = {Galina Zamay and Olga Kolovskaya and Tatiana Ivanchenko and Tatiana Zamay and Dmitry Veprintsev and Valentina Grigorieva and Irina Garanzha and Alexey Krat and Yury Glazyrin and Ana Gargaun and Ivan Lapin and Valery Svetlichnyi and Maxim Berezovski and Anna Kichkailo},
title = {Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood},
journal = {Cancers},
year = {2019},
volume = {11},
publisher = {MDPI},
month = {mar},
url = {https://doi.org/10.3390/cancers11030351},
number = {3},
pages = {351},
doi = {10.3390/cancers11030351}
}
Cite this
MLA
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Zamay, Galina, et al. “Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood.” Cancers, vol. 11, no. 3, Mar. 2019, p. 351. https://doi.org/10.3390/cancers11030351.
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