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MDPI
International Journal of Molecular Sciences
,  volume 20 ,  issue 18 ,  pages 4412

Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion

Publication type: Journal Article
Publication date: 2019-09-07
MDPI
scimago Q1
wos Q1
SJR: 1.273
CiteScore: 9.0
Impact factor: 4.9
ISSN: 16616596, 14220067
DOI:  10.3390/ijms20184412
PubMed ID:  31500317
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract

d-amino acid oxidase (DAAO, EC 1.4.3.3) is used in many biotechnological processes. The main industrial application of DAAO is biocatalytic production of 7-aminocephalosporanic acid from cephalosporin C with a two enzymes system. DAAO from the yeast Trigonopsis variabilis (TvDAAO) shows the best catalytic parameters with cephalosporin C among all known DAAOs. We prepared and characterized multipoint TvDAAO mutants to improve their activity towards cephalosporin C and increase stability. All TvDAAO mutants showed better properties in comparison with the wild-type enzyme. The best mutant was TvDAAO with amino acid changes E32R/F33D/F54S/C108F/M156L/C298N. Compared to wild-type TvDAAO, the mutant enzyme exhibits a 4 times higher catalytic constant for cephalosporin C oxidation and 8- and 20-fold better stability against hydrogen peroxide inactivation and thermal denaturation, respectively. This makes this mutant promising for use in biotechnology. The paper also presents the comparison of TvDAAO catalytic properties with cephalosporin C reported by others.

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GOST Copy
Atroshenko D. L. et al. Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion // International Journal of Molecular Sciences. 2019. Vol. 20. No. 18. p. 4412.
GOST all authors (up to 50) Copy
Atroshenko D. L., Shelomov M. D., Zarubina S. A., Negru N. Y., Golubev I. V., Savin S. S., Tishkov V. I. Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion // International Journal of Molecular Sciences. 2019. Vol. 20. No. 18. p. 4412.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.3390/ijms20184412
UR - https://doi.org/10.3390/ijms20184412
TI - Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion
T2 - International Journal of Molecular Sciences
AU - Atroshenko, Denis L
AU - Shelomov, Mikhail D
AU - Zarubina, Sophia A
AU - Negru, Nikita Y
AU - Golubev, Igor V
AU - Savin, Svyatoslav S
AU - Tishkov, Vladimir I.
PY - 2019
DA - 2019/09/07
PB - MDPI
SP - 4412
IS - 18
VL - 20
PMID - 31500317
SN - 1661-6596
SN - 1422-0067
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2019_Atroshenko,
author = {Denis L Atroshenko and Mikhail D Shelomov and Sophia A Zarubina and Nikita Y Negru and Igor V Golubev and Svyatoslav S Savin and Vladimir I. Tishkov},
title = {Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion},
journal = {International Journal of Molecular Sciences},
year = {2019},
volume = {20},
publisher = {MDPI},
month = {sep},
url = {https://doi.org/10.3390/ijms20184412},
number = {18},
pages = {4412},
doi = {10.3390/ijms20184412}
}
MLA
Cite this
MLA Copy
Atroshenko, Denis L., et al. “Multipoint TvDAAO Mutants for Cephalosporin C Bioconversion.” International Journal of Molecular Sciences, vol. 20, no. 18, Sep. 2019, p. 4412. https://doi.org/10.3390/ijms20184412.