Open Access
International Journal of Molecular Sciences, volume 21, issue 12, pages 4522
Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects
Publication type: Journal Article
Publication date: 2020-06-25
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 16616596, 14220067
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
Aptamers are short single stranded DNA or RNA oligonucleotides that can recognize analytes with extraordinary target selectivity and affinity. Despite their promising properties and diagnostic potential, the number of commercial applications remains scarce. In order to endow them with novel recognition motifs and enhanced properties, chemical modification of aptamers has been pursued. This review focuses on chemical modifications, aimed at increasing the binding affinity for the aptamer’s target either in a non-covalent or covalent fashion, hereby improving their application potential in a diagnostic context. An overview of current methodologies will be given, thereby distinguishing between pre- and post-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) modifications.
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Citations by publishers
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25
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Elskens J. P., Elskens J. M., Madder A. Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects // International Journal of Molecular Sciences. 2020. Vol. 21. No. 12. p. 4522.
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Elskens J. P., Elskens J. M., Madder A. Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects // International Journal of Molecular Sciences. 2020. Vol. 21. No. 12. p. 4522.
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TY - JOUR
DO - 10.3390/ijms21124522
UR - https://doi.org/10.3390/ijms21124522
TI - Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects
T2 - International Journal of Molecular Sciences
AU - Elskens, Jan P
AU - Elskens, Joke M
AU - Madder, Annemieke
PY - 2020
DA - 2020/06/25 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 4522
IS - 12
VL - 21
SN - 1661-6596
SN - 1422-0067
ER -
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@article{2020_Elskens,
author = {Jan P Elskens and Joke M Elskens and Annemieke Madder},
title = {Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects},
journal = {International Journal of Molecular Sciences},
year = {2020},
volume = {21},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jun},
url = {https://doi.org/10.3390/ijms21124522},
number = {12},
pages = {4522},
doi = {10.3390/ijms21124522}
}
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Elskens, Jan P., et al. “Chemical Modification of Aptamers for Increased Binding Affinity in Diagnostic Applications: Current Status and Future Prospects.” International Journal of Molecular Sciences, vol. 21, no. 12, Jun. 2020, p. 4522. https://doi.org/10.3390/ijms21124522.