Open Access
Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose
Nadezda M Podvalnaya
1, 2
,
Varvara V. Dudenkova
3
,
Anna S. Efremova
2
,
Nadya G. Gurskaya
1, 4
,
DMITRY A. GORBACHEV
1
,
Artem V. Luzhin
5
,
Omar L. Kantidze
5
,
Elena V. Zagaynova
3, 6
,
Stanislav I. Shram
2
,
2
Publication type: Journal Article
Publication date: 2020-07-15
scimago Q1
wos Q1
SJR: 1.273
CiteScore: 9.0
Impact factor: 4.9
ISSN: 16616596, 14220067
PubMed ID:
32679873
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
Poly-(ADP-ribosyl)-ation (PARylation) is a reversible post-translational modification of proteins and DNA that plays an important role in various cellular processes such as DNA damage response, replication, transcription, and cell death. Here we designed a fully genetically encoded fluorescent sensor for poly-(ADP-ribose) (PAR) based on Förster resonance energy transfer (FRET). The WWE domain, which recognizes iso-ADP-ribose internal PAR-specific structural unit, was used as a PAR-targeting module. The sensor consisted of cyan Turquoise2 and yellow Venus fluorescent proteins, each in fusion with the WWE domain of RNF146 E3 ubiquitin ligase protein. This bipartite sensor named sPARroW (sensor for PAR relying on WWE) enabled monitoring of PAR accumulation and depletion in live mammalian cells in response to different stimuli, namely hydrogen peroxide treatment, UV irradiation and hyperthermia.
Found
Nothing found, try to update filter.
Found
Nothing found, try to update filter.
Top-30
Journals
|
1
2
|
|
|
DNA Repair
2 publications, 18.18%
|
|
|
Cancers
1 publication, 9.09%
|
|
|
Biochemistry (Moscow)
1 publication, 9.09%
|
|
|
eLife
1 publication, 9.09%
|
|
|
Methods in Molecular Biology
1 publication, 9.09%
|
|
|
bioRxiv
1 publication, 9.09%
|
|
|
Environmental and Molecular Mutagenesis
1 publication, 9.09%
|
|
|
ACS Sensors
1 publication, 9.09%
|
|
|
Neuroscientist
1 publication, 9.09%
|
|
|
1
2
|
Publishers
|
1
2
|
|
|
Elsevier
2 publications, 18.18%
|
|
|
Cold Spring Harbor Laboratory
2 publications, 18.18%
|
|
|
MDPI
1 publication, 9.09%
|
|
|
Pleiades Publishing
1 publication, 9.09%
|
|
|
eLife Sciences Publications
1 publication, 9.09%
|
|
|
Springer Nature
1 publication, 9.09%
|
|
|
Wiley
1 publication, 9.09%
|
|
|
American Chemical Society (ACS)
1 publication, 9.09%
|
|
|
SAGE
1 publication, 9.09%
|
|
|
1
2
|
- We do not take into account publications without a DOI.
- Statistics recalculated weekly.
Are you a researcher?
Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
11
Total citations:
11
Citations from 2024:
5
(45.45%)
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Serebrovskaya E. O. et al. Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose // International Journal of Molecular Sciences. 2020. Vol. 21. No. 14. p. 5004.
GOST all authors (up to 50)
Copy
Serebrovskaya E. O., Podvalnaya N. M., Dudenkova V. V., Efremova A. S., Gurskaya N. G., GORBACHEV D. A., Luzhin A. V., Kantidze O. L., Zagaynova E. V., Shram S. I., Lukyanov K. A. Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose // International Journal of Molecular Sciences. 2020. Vol. 21. No. 14. p. 5004.
Cite this
RIS
Copy
TY - JOUR
DO - 10.3390/ijms21145004
UR - https://doi.org/10.3390/ijms21145004
TI - Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose
T2 - International Journal of Molecular Sciences
AU - Serebrovskaya, Ekaterina O.
AU - Podvalnaya, Nadezda M
AU - Dudenkova, Varvara V.
AU - Efremova, Anna S.
AU - Gurskaya, Nadya G.
AU - GORBACHEV, DMITRY A.
AU - Luzhin, Artem V.
AU - Kantidze, Omar L.
AU - Zagaynova, Elena V.
AU - Shram, Stanislav I.
AU - Lukyanov, Konstantin A.
PY - 2020
DA - 2020/07/15
PB - MDPI
SP - 5004
IS - 14
VL - 21
PMID - 32679873
SN - 1661-6596
SN - 1422-0067
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2020_Serebrovskaya,
author = {Ekaterina O. Serebrovskaya and Nadezda M Podvalnaya and Varvara V. Dudenkova and Anna S. Efremova and Nadya G. Gurskaya and DMITRY A. GORBACHEV and Artem V. Luzhin and Omar L. Kantidze and Elena V. Zagaynova and Stanislav I. Shram and Konstantin A. Lukyanov},
title = {Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose},
journal = {International Journal of Molecular Sciences},
year = {2020},
volume = {21},
publisher = {MDPI},
month = {jul},
url = {https://doi.org/10.3390/ijms21145004},
number = {14},
pages = {5004},
doi = {10.3390/ijms21145004}
}
Cite this
MLA
Copy
Serebrovskaya, Ekaterina O., et al. “Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose.” International Journal of Molecular Sciences, vol. 21, no. 14, Jul. 2020, p. 5004. https://doi.org/10.3390/ijms21145004.