Open Access
International Journal of Molecular Sciences, volume 21, issue 14, pages 5004
Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose
Podvalnaya Nadezda M
1, 2
,
Dudenkova Varvara V.
3
,
Efremova Anna S.
2
,
Gurskaya Nadya G.
1, 4
,
GORBACHEV DMITRY A.
1
,
Luzhin Artem V.
5
,
Kantidze Omar L.
5
,
Zagaynova Elena V.
3, 6
,
Shram Stanislav I.
2
,
2
Publication type: Journal Article
Publication date: 2020-07-15
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 16616596, 14220067
PubMed ID:
32679873
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
Poly-(ADP-ribosyl)-ation (PARylation) is a reversible post-translational modification of proteins and DNA that plays an important role in various cellular processes such as DNA damage response, replication, transcription, and cell death. Here we designed a fully genetically encoded fluorescent sensor for poly-(ADP-ribose) (PAR) based on Förster resonance energy transfer (FRET). The WWE domain, which recognizes iso-ADP-ribose internal PAR-specific structural unit, was used as a PAR-targeting module. The sensor consisted of cyan Turquoise2 and yellow Venus fluorescent proteins, each in fusion with the WWE domain of RNF146 E3 ubiquitin ligase protein. This bipartite sensor named sPARroW (sensor for PAR relying on WWE) enabled monitoring of PAR accumulation and depletion in live mammalian cells in response to different stimuli, namely hydrogen peroxide treatment, UV irradiation and hyperthermia.
Citations by journals
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1
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Cancers
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Cancers
1 publication, 16.67%
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DNA Repair
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DNA Repair
1 publication, 16.67%
|
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Biochemistry (Moscow)
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Biochemistry (Moscow)
1 publication, 16.67%
|
|
eLife
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eLife
1 publication, 16.67%
|
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Methods in Molecular Biology
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Methods in Molecular Biology
1 publication, 16.67%
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|
1
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Citations by publishers
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1
|
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 16.67%
|
|
Elsevier
|
Elsevier
1 publication, 16.67%
|
|
Pleiades Publishing
|
Pleiades Publishing
1 publication, 16.67%
|
|
eLife Sciences Publications
|
eLife Sciences Publications
1 publication, 16.67%
|
|
Springer Nature
|
Springer Nature
1 publication, 16.67%
|
|
1
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Serebrovskaya E. O. et al. Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose // International Journal of Molecular Sciences. 2020. Vol. 21. No. 14. p. 5004.
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Serebrovskaya E. O., Podvalnaya N. M., Dudenkova V. V., Efremova A. S., Gurskaya N. G., GORBACHEV D. A., Luzhin A. V., Kantidze O. L., Zagaynova E. V., Shram S. I., Lukyanov K. A. Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose // International Journal of Molecular Sciences. 2020. Vol. 21. No. 14. p. 5004.
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TY - JOUR
DO - 10.3390/ijms21145004
UR - https://doi.org/10.3390%2Fijms21145004
TI - Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose
T2 - International Journal of Molecular Sciences
AU - Podvalnaya, Nadezda M
AU - Dudenkova, Varvara V.
AU - Efremova, Anna S.
AU - GORBACHEV, DMITRY A.
AU - Zagaynova, Elena V.
AU - Shram, Stanislav I.
AU - Serebrovskaya, Ekaterina O.
AU - Gurskaya, Nadya G.
AU - Luzhin, Artem V.
AU - Kantidze, Omar L.
AU - Lukyanov, Konstantin A.
PY - 2020
DA - 2020/07/15 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 5004
IS - 14
VL - 21
PMID - 32679873
SN - 1661-6596
SN - 1422-0067
ER -
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@article{2020_Serebrovskaya,
author = {Nadezda M Podvalnaya and Varvara V. Dudenkova and Anna S. Efremova and DMITRY A. GORBACHEV and Elena V. Zagaynova and Stanislav I. Shram and Ekaterina O. Serebrovskaya and Nadya G. Gurskaya and Artem V. Luzhin and Omar L. Kantidze and Konstantin A. Lukyanov},
title = {Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose},
journal = {International Journal of Molecular Sciences},
year = {2020},
volume = {21},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jul},
url = {https://doi.org/10.3390%2Fijms21145004},
number = {14},
pages = {5004},
doi = {10.3390/ijms21145004}
}
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MLA
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Serebrovskaya, Ekaterina O., et al. “Genetically Encoded Fluorescent Sensor for Poly-ADP-Ribose.” International Journal of Molecular Sciences, vol. 21, no. 14, Jul. 2020, p. 5004. https://doi.org/10.3390%2Fijms21145004.