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Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency

Тип публикацииJournal Article
Дата публикации2023-03-12
scimago Q1
wos Q1
БС1
SJR1.273
CiteScore9.0
Impact factor4.9
ISSN16616596, 14220067
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Краткое описание

Ocular surface reconstruction is essential for treating corneal epithelial defects and vision recovery. Stem cell-based therapy demonstrates promising results but requires further research to elucidate stem cell survival, growth, and differentiation after transplantation in vivo. This study examined the corneal reconstruction promoted by EGFP-labeled limbal mesenchymal stem cells (L-MSCs-EGFP) and their fate after transplantation. EGFP labeling allowed us to evaluate the migration and survival rates of the transferred cells. L-MSCs-EGFP seeded onto decellularized human amniotic membrane (dHAM) were transplanted into rabbits with a modeled limbal stem cell deficiency. The localization and viability of the transplanted cells in animal tissue were analyzed using histology, immunohistochemistry, and confocal microscopy up to 3 months after transplantation. EGFP-labeled cells remained viable for the first 14 days after transplantation. By the 90th day, epithelialization of the rabbit corneas reached 90%, but the presence of viable labeled cells was not observed within the newly formed epithelium. Although labeled cells demonstrated low survivability in host tissue, the squamous corneal-like epithelium was partially restored by the 30th day after transplantation of the tissue-engineered graft. Overall, this study paves the way for further optimization of transplantation conditions and studying the mechanisms of corneal tissue restoration.

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Experimental Eye Research
2 публикации, 33.33%
Medicina
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Survey of Ophthalmology
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International Journal of Molecular Sciences
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Acta Biomaterialia
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Elsevier
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ГОСТ |
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Khorolskaya J. I. et al. Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency // International Journal of Molecular Sciences. 2023. Vol. 24. No. 6. p. 5431.
ГОСТ со всеми авторами (до 50) Скопировать
Khorolskaya J. I., Perepletchikova D. A., Zhurenkov K. E., Kachkin D. V., Rubel A. A., Blinova M. I., Mikhailova N. A. Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency // International Journal of Molecular Sciences. 2023. Vol. 24. No. 6. p. 5431.
RIS |
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TY - JOUR
DO - 10.3390/ijms24065431
UR - https://doi.org/10.3390/ijms24065431
TI - Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency
T2 - International Journal of Molecular Sciences
AU - Khorolskaya, Julia I.
AU - Perepletchikova, Daria A
AU - Zhurenkov, Kirill E.
AU - Kachkin, Daniel V
AU - Rubel, Aleksandr A.
AU - Blinova, Miralda I.
AU - Mikhailova, Natalia A
PY - 2023
DA - 2023/03/12
PB - MDPI
SP - 5431
IS - 6
VL - 24
PMID - 36982507
SN - 1661-6596
SN - 1422-0067
ER -
BibTex |
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BibTex (до 50 авторов) Скопировать
@article{2023_Khorolskaya,
author = {Julia I. Khorolskaya and Daria A Perepletchikova and Kirill E. Zhurenkov and Daniel V Kachkin and Aleksandr A. Rubel and Miralda I. Blinova and Natalia A Mikhailova},
title = {Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency},
journal = {International Journal of Molecular Sciences},
year = {2023},
volume = {24},
publisher = {MDPI},
month = {mar},
url = {https://doi.org/10.3390/ijms24065431},
number = {6},
pages = {5431},
doi = {10.3390/ijms24065431}
}
MLA
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Khorolskaya, Julia I., et al. “Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency.” International Journal of Molecular Sciences, vol. 24, no. 6, Mar. 2023, p. 5431. https://doi.org/10.3390/ijms24065431.