Open Access
International Journal of Molecular Sciences, volume 24, issue 14, pages 11725
Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms
Kostyukevich Yury
1
,
Stekolshikova Elena
1
,
Levashova Anna
1, 2
,
Kovalenko Anna
1
,
Vishnevskaya Anna
1
,
Bashilov Anton
1
,
Kireev Albert
1
,
Khaitovich Philipp
1
,
Osipenko Sergey
1
,
Nikolaev Evgeny
1
Publication type: Journal Article
Publication date: 2023-07-21
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 5.6
ISSN: 16616596, 14220067
PubMed ID:
37511483
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract
The administration of low doses of D2O to living organisms was used for decades for the investigation of metabolic pathways and for the measurement of the turnover rate for specific compounds. Usually, the investigation of the deuterium uptake in lipids is performed by measuring the deuteration level of the palmitic acid residue using GC-MS instruments, and to our knowledge, the application of the modern untargeted LC-MS/MS lipidomics approaches was only reported a few times. Here, we investigated the deuterium uptake for >500 lipids for 13 organs and body liquids of mice (brain, lung, heart, liver, kidney, spleen, plasma, urine, etc.) after 4 days of 100% D2O administration. The maximum deuteration level was observed in the liver, plasma, and lung, while in the brain and heart, the deuteration level was lower. Using MS/MS, we demonstrated the incorporation of deuterium in palmitic and stearic fragments in lipids (PC, PE, TAG, PG, etc.) but not in the corresponding free forms. Our results were analyzed based on the metabolic pathways of lipids.
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International Journal of Molecular Sciences
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International Journal of Molecular Sciences
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Citations by publishers
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 100%
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Kostyukevich Y. et al. Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms // International Journal of Molecular Sciences. 2023. Vol. 24. No. 14. p. 11725.
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Kostyukevich Y., Stekolshikova E., Levashova A., Kovalenko A., Vishnevskaya A., Bashilov A., Kireev A., Tupertsev B. S., Rumiantseva L., Khaitovich P., Osipenko S., Nikolaev E. Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms // International Journal of Molecular Sciences. 2023. Vol. 24. No. 14. p. 11725.
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TY - JOUR
DO - 10.3390/ijms241411725
UR - https://doi.org/10.3390%2Fijms241411725
TI - Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms
T2 - International Journal of Molecular Sciences
AU - Kostyukevich, Yury
AU - Stekolshikova, Elena
AU - Levashova, Anna
AU - Kovalenko, Anna
AU - Vishnevskaya, Anna
AU - Bashilov, Anton
AU - Kireev, Albert
AU - Tupertsev, Boris S
AU - Rumiantseva, Lidiia
AU - Khaitovich, Philipp
AU - Osipenko, Sergey
AU - Nikolaev, Evgeny
PY - 2023
DA - 2023/07/21 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 11725
IS - 14
VL - 24
PMID - 37511483
SN - 1661-6596
SN - 1422-0067
ER -
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@article{2023_Kostyukevich,
author = {Yury Kostyukevich and Elena Stekolshikova and Anna Levashova and Anna Kovalenko and Anna Vishnevskaya and Anton Bashilov and Albert Kireev and Boris S Tupertsev and Lidiia Rumiantseva and Philipp Khaitovich and Sergey Osipenko and Evgeny Nikolaev},
title = {Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms},
journal = {International Journal of Molecular Sciences},
year = {2023},
volume = {24},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {jul},
url = {https://doi.org/10.3390%2Fijms241411725},
number = {14},
pages = {11725},
doi = {10.3390/ijms241411725}
}
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Kostyukevich, Yury, et al. “Untargeted Lipidomics after D2O Administration Reveals the Turnover Rate of Individual Lipids in Various Organs of Living Organisms.” International Journal of Molecular Sciences, vol. 24, no. 14, Jul. 2023, p. 11725. https://doi.org/10.3390%2Fijms241411725.