Open Access
Materials, volume 13, issue 3, pages 677
A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets
1
Laboratory of Animal Genetics and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
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2
Division of Materials Science, Graduate School of Environmental Science, Hokkaido University, Sapporo 0860-0810, Japan
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3
Laboratory of Animal Genetics and Reproduction, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan
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4
Graduate School of Global Food Resources/Global Station for Food, Land and Water Resources, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo 060-8589, Japan
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Publication type: Journal Article
Publication date: 2020-02-03
PubMed ID:
32028677
General Materials Science
Abstract
We developed an easily accessible boron-dipyrromethene (BODIPY)-based fluorogenic probe, which we named LD-TB. This probe emits bright fluorescence in oil; when compared with aqueous solution, a significant enhancement of fluorescence brightness is observed. Cellular experiments confirmed that the probe stains the lipid droplets (LDs) specifically in both live and fixed cells, providing background-free images. Compared with Nile Red dye, a commonly used LD marker, LD-TB showed superior photostability. The sharp absorption and emission bands enable its multicolor imaging with blue and green probes. Importantly, the probe has proved to have low toxicity and is compatible with cell fixation. Our research provides a promising new fluorogenic probe for specific imaging of LDs.
Citations by journals
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Molecules
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Citations by publishers
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1
2
3
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5
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Multidisciplinary Digital Publishing Institute (MDPI)
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5 publications, 31.25%
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Elsevier
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Elsevier
2 publications, 12.5%
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Springer Nature
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Springer Nature
2 publications, 12.5%
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Wiley
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Wiley
2 publications, 12.5%
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Hindawi Limited
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Hindawi Limited
1 publication, 6.25%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
1 publication, 6.25%
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Cold Spring Harbor Laboratory
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Cold Spring Harbor Laboratory
1 publication, 6.25%
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American Institute of Physics (AIP)
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American Institute of Physics (AIP)
1 publication, 6.25%
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1
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5
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Li et al. A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets // Materials. 2020. Vol. 13. No. 3. p. 677.
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Li, Otsuka, Zhang, Takahashi, Yamada A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets // Materials. 2020. Vol. 13. No. 3. p. 677.
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TY - JOUR
DO - 10.3390/ma13030677
UR - https://doi.org/10.3390%2Fma13030677
TI - A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets
T2 - Materials
AU - Li
AU - Otsuka
AU - Zhang
AU - Takahashi
AU - Yamada
PY - 2020
DA - 2020/02/03 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 677
IS - 3
VL - 13
PMID - 32028677
SN - 1996-1944
ER -
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@article{2020_Li,
author = {Li and Otsuka and Zhang and Takahashi and Yamada},
title = {A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets},
journal = {Materials},
year = {2020},
volume = {13},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {feb},
url = {https://doi.org/10.3390%2Fma13030677},
number = {3},
pages = {677},
doi = {10.3390/ma13030677}
}
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MLA
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Li, et al. “A BODIPY-Based Fluorogenic Probe for Specific Imaging of Lipid Droplets.” Materials, vol. 13, no. 3, Feb. 2020, p. 677. https://doi.org/10.3390%2Fma13030677.